摘要
目的观察通经补肾中药方对铝诱导的AD模型大鼠海马组织中乙酰胆碱酯酶(AchE)和胆碱乙酰转移酶(ChAT)活性及乙酰胆碱(Ach)含量变化。方法选择健康3月龄SD大鼠84只,随机分为对照组、模型组(分为低、中、高剂量铝饲料组)、中药组(分为低、中、高剂量中药组),每组各12只。模型组在常规饲料中添加不同剂量A1C13.6H2O喂饲大鼠,持续染毒3个月,制作AD动物模型。中药组大鼠喂饲含铝饲料,并以通经补肾中药方灌胃。上述实验结束后,处死大鼠,取海马组织,测定海马中的AchE和ChAT的活性及Ach含量。结果与对照组比较,中、高剂量铝饲料组大鼠海马中AchE的活性明显增高(P<0.01),ChAT活性明显降低(P<0.01),Ach含量显著减少(P<0.01)。与高剂量铝饲料组比较,中、高剂量中药组大鼠海马中AchE活性明显降低(P<0.01),ChAT活性明显升高(P<0.01),Ach含量明显增加(P<0.01)。结论铝的过多摄入使大鼠海马Ach减少可能是AD发病的机制之一,中药方可提高海马中ChAT活性,抑制AchE活性,增加Ach的含量,改善大鼠中枢胆碱能系统失衡状况。
Objective To observe the effect of Tongjingbushen complex prescription on acetylcholine esterase （AchE）, choline acetyhransferase （CHAT） and acetylcholine （Ach） in hippocampus of Alzheimer＇s disease （AD） rat induced by aluminum. Methods 84 3-month-old SD rats were divided into control, model （ low, middle and high dose of aluminum）, traditional Chinese medicine （ low, middle and high dose of traditional Chinese medicine） groups （n = 12）. Rats of AD were made by adding A1C13 · 6H2O to normal feed for 3 months. Rats in traditional Chinese medicine groups were given Tongjingbushen traditional Chinese medicine i. g. AchE, ChAT and Ach in hippocampus were detected. Results Compared with control group, AchE activity were higher, ChAT activity were decreased, Ach were increased in middle and high dose model groups （ P 〈 0. 01 ）. Conclusions More uptake of aluminum resulting Ach decrease may be one of nosogenesis of AD,traditional Chinese medicine could increase ChAT activity, inhibit Ache activity, increase Ach content in hippocampus, improve rat central cholinergic system unbalance.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第21期4673-4674,共2页
Chinese Journal of Gerontology
基金
河北省科技支撑计划项目(No.06276102-56)
关键词
铝
阿尔茨海默病
通经补肾复方
乙酰胆碱酯酶
胆碱乙酰转移酶
乙酰胆碱
Aluminum
Alzheimer＇s disease
Tongjingbushen complex prescription
Acetylcholine esterase
Choline acetyltrans-ferase
Acetylcholine
