摘要
目的:探讨淫羊藿苷(ICA)对快速老化小鼠SAMP10脑组织单胺类及氨基酸类神经递质的影响。方法:本实验采取8月龄快速老化小鼠SAMP10为实验对象,随机分为模型SAMP10组、阳性药多奈哌齐组(1mg/kg)、ICA 3个剂量(50、100、200mg/kg)组,每组12只,以12只同月龄抗快速老化小鼠SAMR1为正常对照。灌胃给药30d,一天一次,高效液相-电化学法检测快速老化小鼠SAMP10大脑皮层的去甲肾上腺素(NE)、5-羟色胺(5-HT)、多巴胺(DA)及其代谢产物3,4-二羟苯乙酸(DOPAC)和高香草酸(HVA)的含量来探讨ICA对SAMP10脑组织单胺类神经递质的影响,通过检测谷氨酸(Glu)、谷氨酰胺(Gln)、天冬氨酸(Asp)、γ-氨基丁酸(GA-BA)、牛磺酸(Tau)、甘氨酸(Gly)的含量来探讨ICA对SAMP10脑组织氨基酸类神经递质的影响。结果:ICA可显著降低SAMP10大脑皮层内Glu、Gln、GABA含量(P<0.01),升高NE、DA、DOPAC、5-HT、HVA、Asp以及Tau的含量(P<0.01或P<0.05),但对SAMP10大脑皮层内Gly的含量并没有显著影响(P>0.05)。结论:ICA可能通过升高脑内单胺类神经递质的含量、调节兴奋性氨基酸类递质的代谢平衡以及调节抑制性氨基酸的代谢平衡达到改善SAMP10的学习记忆的作用。
AIM： To investigate the effects of icariin（ICA） on monoamine and amino acid neurotransmitters in senescence accelerated mouse prone/10（SAMP10）.METHODS： The 8-month-old SAMP10 were randomly divided into model group,Donepezil Hydrochloride（DON）-treated SAMP10 group（1 mg/kg） and ICA-treated SAMP10 groups（50,100,200 mg/kg）,with twelve mice in each group.Twelve senescence accelerated mouse/resistance1（SAMR1） with the same age were used for normal control.After 30 days of oral administration,HPLC with electrochemical detection（EC） was used to measure norepinephrine,5-hydroxytryptamine,dopamine and its metabolites 3,4-phenyl acetic acid and homovanillic acid,and glutamic acid,glutamine,aspartic acid,γ-aminobutyric acid,taurine,glycine.RESULTS：ICA could significantly reduce the levels of glutamic acid,glutamine and γ-aminobutyric acid in the cerebral cortex of SAMP10（P0.01）,and increase the levels of norepinephrine,dopamine,3,4-phenyl acetic acid,5-hydroxytryptamine,homovanillic acid,aspartic acid and taurine（P0.01 or P0.05）,but glycine had no significant effect（P0.05）.CONCLUSION： The improvement of ICA on the ability of learning and memory in SAMP10 may be related to elevating levels of monoamine neurotransmitter,regulating the balance of excitatory amino acids and regulating the balance of inhibitory amino acids.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2012年第10期1081-1086,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金项目(30772750)
"重大新药创制"科技重大专项(2009ZX09301-003)
