摘要
背景:角膜碱烧伤后损伤修复受许多因素的影响,热休克蛋白可促进变性、损伤蛋白质的迅速恢复或清除。目的:观察大鼠角膜碱烧伤后热休克蛋白70的表达及其与角膜损伤修复的关系。方法:检查大鼠眼无炎症及其他病变后,奥布卡因滴眼液点眼2次,棉签吸除结膜囊液体,将统一规格直径5mm的滤纸片浸泡于1mol/L NaOH溶液中10s,然后置于大鼠角膜中央30s制作大鼠角膜碱烧伤模型。分别于碱烧伤后6h,1,3,7,14,21d取材。结果与结论:RT-PCR、免疫组织化学染色、Western blot结果均显示热休克蛋白70mRNA和蛋白在角膜碱烧伤后1d即开始升高,7d时达高峰,14d后开始下降。苏木精-伊红染色及电镜观察显示角膜损伤在烧伤后6h即较明显,烧伤后7d逐渐恢复。提示大鼠角膜碱烧伤后热休克蛋白70的表达与碱烧伤后角膜损伤修复过程一致,参与了大鼠角膜碱烧伤后细胞的自我保护及修复过程。
BACKGROUND：Many factors can influence the wound healing of cornea following alkali burn, heat shock protein can promote the rapidly recovery and clear of the degenerated and injured protein. OBJECTIVE：To investigate whether the expression of heat shock protein 70 after corneal alkali burn in rats is related to corneal wound healing process. METHODS：After the rat eye was checked without inflammation and other lesions, oxybuprocaine eye drops were dropped into the rat eye for twice. Conjunctival sac liquid was sucked by swab, and then the unified specification filter paper of 5 mm diameter was soaked in 1 mol/L NaOH solution for 10 seconds, then the filter paper was placed in the center of the rat cornea for 30 seconds to make the rat corneal alkali burn model. The corneal specimens were obtained at 6 hours, 1, 3, 7, 14 and 21 days after alkali burn. RESULTS AND CONCLUSION：Reverse transcription polymerase chain reaction, immunohistochemistry staining and Western blot observation showed that the expression level of heat shock protein 70 increased at 1 day after alkali burn and reached its peak at 7 days. Then, it decreased at 14 days. Hematoxylin-eosin staining and transmission electron microscope observation showed that the corneal injury was most obvious at 6 hours after burning and the wound was gradually recovered at 7 days after burning. Alkali burned cornea can induce heat shock protein 70 synthesis in a time-dependent manner, also in accordance with the corneal repair procedure. Induced heat shock protein 70 plays an important role in wound healing.
出处
《中国组织工程研究》
CAS
CSCD
2012年第37期6973-6978,共6页
Chinese Journal of Tissue Engineering Research
基金
the National Natural Science Foundation of China,No.30901658~~
