摘要
为探讨高胆固醇喂养对兔肝细胞膜高密度脂蛋白受体的影响 ,用高胆固醇饲料喂养家兔 6个月后剖杀 ,取肝脏用蔗糖密度梯度超速离心法分离肝细胞膜 ,用密度梯度超速离心法分离普通饲料喂养的家兔血浆不含载脂蛋白E的高密度脂蛋白 3 ,用氯化单碘法对兔高密度脂蛋白 3进行1 2 5 I标记。以1 2 5 I标记的兔高密度脂蛋白 3作为配体 ,对肝细胞膜高密度脂蛋白受体进行研究。结果发现 ,高胆固醇饲料和普通饲料喂养的家兔肝细胞膜高密度脂蛋白受体的最大结合分别为 2 .46± 0 .72mg g和 1.18± 0 .48mg g ,两者差异显著 (P <0 .0 0 1) ,但解离常数值无显著性改变 ( 8.0 6± 3.10mg L比 9.2 7± 3.5 4mg L ,P >0 .0 5 )。该结果表明高胆固醇饲养能上升调节家兔高密度脂蛋白受体 。
Aim To investigate the effect of high cholesterol chow on high density lipoprotein (HDL) receptors in hepatic plasma membranes with 125 I-labeled HDL 3 as ligands. Methods The hepatic plasma membranes were purified by density gradient ultracentrifugation from rabbits fed with high cholesterol chow and rabbits fed with normal chow. Apolipoprotein E deficient HDL 3 was isolated from plasma of normally fed rabbits by density gradient ultracentrifugation, and labeled with 125 I by ICl method. Radioreceptor assays were carried out by incubation of the purified plasma membranes with labeled and unlabeled HDL. Results HDL receptors on hepatic plasma membranes of rabbits fed with high cholesterol chow and normal chow have an average Kd of 8.06±3.10 mg/L and 9.27±3.54 mg/L. There is no statistical difference of Kd between the two groups. But HDL receptors on hepatic plasma membranes of rabbits fed with high cholesterol chow have an higher value of Bmax than those with normal chow (2.46±0.72 mg/g vs 1.18±0.48 mg/g, P<0.001). Conclusions The high cholesterol chow can up-regulate the HDL receptors, and increase the HDL receptor activity on hepatic plasma membranes of rabbits.
出处
《中国动脉硬化杂志》
CAS
CSCD
2000年第2期140-142,共3页
Chinese Journal of Arteriosclerosis
