摘要
目的 研究诱导分化及糖基化终产物 (AGEs)对人单核 /巨噬细胞 (U937细胞 )高密度脂蛋白受体 (SR BI)蛋白表达的影响 ,探讨AGEs和巨噬细胞SR BI在动脉粥样硬化中的作用。方法 U937细胞经PMA诱导分化 ,并将不同浓度或同一浓度AGEs与诱导分化 48h后的U937细胞共同孵育 ,用免疫细胞化学法和Western印迹法检测SR BI蛋白的表达。结果 诱导分化后U937细胞SR BI表达在 2 4、48h逐渐升高 ,72h下降 ;1 0 0、2 0 0和 40 0 μg/mlAGEs刺激后细胞表面SR BI蛋白表达量分别是BSA组的 1 44、2 38和 2 77倍 (P <0 0 5) ;40 0 μg/ml的AGEs作用 6、1 2、2 4、48h后 ,U937巨噬细胞SR BI蛋白表达量分别为 0h的 1 38、2 49、3 76和 4 2 5倍 (P <0 0 5)。结论 AGEs可增加U937巨噬细胞SR BI蛋白表达且呈浓度和时间依赖性。
Objective To research the effects of dif fe rentiation and advanced glycosylation end products (AGEs) on expression of scave nger receptor class B typeⅠ(SR-BI) in U937 macrophages and explore the functio n of AGEs and SR-BI in atherosclerosis. Methods Differ entiation of U937 cell was induced by PMA, and the U937 cells after differentiat ion for 48h were incubated in medium with AGEs in different concentration or the same concentration. Immunocytochemical method and Western blotting analysis wer e used to detect SR-BI protein expression. Results SR -BI protein in U937 was increased after differentiation, reached a peak on 48 h , and decreased on 72h. Expression of SR-BI protein was 1.44, 2.38 and 2.77 ti mes in 100 μg/ml, 200 μg/ml and 400μg/ml AGEs groups as many as that in BSA g roup (P<0.05); SR-BI expression in U937 macrophages following 400μg/ml AGEs for 6 h, 12 h, 24 h, 48 h was increased by 1.38, 2.49, 3.76 and 4.25 tim es compared with control group (P<0 05). Conclusions AGEs could increase the expression of SR-BI protein in a time and dose de pendent manner in U937 macrophages.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2004年第11期1041-1043,共3页
Chinese Journal of Gerontology
