摘要
为了建立金标记快速检测试纸条检测食品中四环素(TC)残留的新方法,用硝酸纤维素微孔滤膜(NC膜)作为包被材料;实验室自制金标记抗TC单抗,选择原溶胶40%制备金标记抗体溶胶;以TC-BSA包被检测线(T线),包被浓度选择1.5 mg/mL;实验室自制兔抗鼠IgG包被质控线(C线),包被浓度选择2.5 mg/mL.该试纸条灵敏度可达50 ng/mL,与酶联免疫法试剂做对比,100份牛奶样品的灵敏度等各项指标符合率为100%.该试纸条具有质量优良、操作简便、不需仪器设备、保质期长等特点,适用于食用动物养殖场、食品加工企业和食品进出口检验检疫等单位食品中TC的快速检测.
To establish the TC rapid detection strip with colloidal gold labeling for detecting on tetracycline（TC） residue in food,coating material is made of nitrocellulose filters,colloidal gold labeling monoclonal antibody against tetracycline（TC mAb）is made from the lab by ourselves.Colloidal gold labeling TC mAb collosol is 40% of its original.Test line（T line）is coated with TC-BSA of 1.5 mg/mL,while Control line（C line）is coated with rabbit anti-rat IgG of 2.5 mg/mL.This test strip sensitivity is 50 ng/mL.The novel method coincidence rate is 100% compared with ELISA in 100 milk samples.This strip is suitable for the TC rapid detection in food animal farm,food processing plant,food entry-exit inspection and quarantine system.
出处
《河南科学》
2012年第10期1462-1465,共4页
Henan Science
关键词
四环素残留
金标记
试纸条
快速检测
tetracycline residue
colloidal gold labeling
test strip
rapid detection
