摘要
目的探讨膀胱尿路上皮癌中Notch4的表达情况和微血管密度(MVD)水平。方法选取85例膀胱尿路上皮癌和27例癌旁组织制成组织芯片,Envision两步法检测组织芯片Notch4和CD34的表达。Notch4的表达采用半定量分析,MVD的计数采用Weidner法。结果 (1)Notch4在膀胱尿路上皮癌中呈高表达,癌旁组织中呈低表达。膀胱尿路上皮癌与癌旁组织比较,差别有统计学意义(P<0.05)。(2)MVD随着膀胱尿路上皮癌临床分期、病理分级的增高而增高(P<0.05);与患者年龄、肿瘤数目,是否复发均无显著相关性(P>0.05)。(3)Notch4阳性表达的尿路上皮癌其MVD值明显高于阴性表达组,且差别有统计学意义(P<0.05)。结论 Notch4的高表达与膀胱尿路上皮癌血管生成密切相关,阻断Notch4信号通路有望成为膀胱尿路上皮癌抗血管治疗的潜在靶点。
Objective To investigate the expression and clinical signicance of microvessel density（MVD） and Notch4 in bladder urothelial carcinoma.Methods Paraffin-embeded specimen from 85 cases of bladder urothelial carcinoma and 27 cases of normal tissues near the bladder urothelial carcinoma,which were all filed with full pathological and clinical data,were collected from the pathology department of the First Affiliated Hospital of Fujian Medical University between 2008 and 2010.The expression of CD34 and Notch4 was detected by immunohistochemical with tissue microarray.Notch4 was conducted by semiquantitative analysis and MVD determination was performed according to the method described by Weidner.Results First,the expression of Notch4 in bladder urothelial carcinoma was significantly higher than that in normal tissues（P0.05）.Second,the higher was clinical stage and pathologic grade,the higher was MVD in the bladder urothelial carcinoma（P0.05）.MVD had no correlation with age,tumor numbers and recurrence of the neoplasm（P0.05）.Third,MVD of the Notch4 positive was more densitive than that of Notch4 negtive in uroepithelial carcinoma.Conclusion The higher expression of Notch4 in bladder maybe associated with angiogenesis of urothelial carcinoma.Blocking Notch4 signaling maybe inhibit the development of bladder urothelial carcinoma,and became a potencial target for anti-angiogenic therapy.
出处
《福建医科大学学报》
2012年第5期319-322,共4页
Journal of Fujian Medical University
基金
福建省卫生厅青年科研课题(2009-1-15)
关键词
膀胱
膀胱肿瘤
尿道肿瘤
毛细血管
芯片分析技术
免疫组织化学
遗传学
urinary bladder
urinary bladder neoplasms
urethral neoplasms
capillaries
Microchip Analytical Procedures
Immunohistochemistry
genetics
