摘要
目的评价颊细胞微核实验检测口腔扁平苔藓(OLP)DNA损伤及临床药物治疗的效果。方法临床收集OLP患者39例,并按其口腔表现分为网纹型、红斑型、溃疡型三组。针对网纹型局部给予维甲酸;红斑型口服硫酸羟氯喹结合含漱他克莫司;溃疡型局部注射曲安奈德结合含漱他克莫司。药物治疗为期4周。于治疗前后分别进行REU评分以及颊细胞微核实验,从而评价临床药物治疗效果。结果 OLP病损处的微核率明显高于正常对照。药物治疗4周后三组的REU评分均明显降低,但仅溃疡型组微核率明显降低,其余二组微核率的降低差异无统计学意义。结论 OLP的DNA损伤率高于正常黏膜;局部注射曲安奈德能降低糜烂型OLP的DNA损伤率,可能有助于减少其恶变倾向。
Objective The aim of this study was to determine the recent DNA injury and the therapeutic effect of medicine in oral lichen planus using buccal micronucleus cytome assay. Methods Total 39 patients with a clinicopathological diagnosis of oral lichen planus （including three clinical forms： reticular, erythematous, ulcerative） are given 4-weeks treatment： topical retinoids for reticular forms, Hydroxyehloroquine and topical tacrolimus gargle for erythematous forms and intralesional triamcinolone acetonide （TA） injection and topical tacrolimus gargle for ulcerative forms. Then buccal micronucleus cytome assay and REU scoring before and after the treatment were used to determine the therapeutic effect of medicine in oral lichen planus. Results MEC frequency in the three forms of OLP lesions was significantly elevated （P 〈 0.01 ） compared to that in adjacent normal mucosa. REU scoring in the three forms of OLP lesions was reduced （P 〈 0.01 ） after clinic treatments. Only MEC frequency in ulcerative forms of OLP lesions treated with Intralesional TA injection was significantly reduced （P 〈0.01 ）. Conclusions Medicine treatment can release the symptom of the patients with OLP. Intralesional TA injection can reduce DNA injury in oral lichen planus, and to reduce the risk of developing into cancer.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2012年第5期29-32,共4页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
国家自然科学基金青年项目(81200787)
广东省科技计划(2006B36006010)
中山大学青年教师培育项目(11ykpy55)
关键词
微核
口腔扁平苔藓
Micronucleus
Oral lichen planus
