摘要
目的初步确定太白楤木抗肝损伤作用的药效物质基础。方法采用经典的系统溶剂法(极性由小到大:石油醚-氯仿-乙酸乙酯-正丁醇)依次对太白楤木提取液进行萃取,将各提取液分别灌胃给予四氯化碳(CCl4)致急性肝损伤模型小鼠,检测血清及肝组织中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)的活性和丙二醛(MDA)、谷胱苷肽过氧化物酶(GSH-Px)的水平,比较各部位的抗肝损伤作用;将筛选出的活性部位用大孔吸附树脂进行分离纯化,不同浓度乙醇进行梯度洗脱,洗脱液分别灌胃给予肝损伤模型小鼠,检测血清及肝组织中ALT、AST、SOD的活性和MDA、GSH-Px的水平,比较各洗脱部位的抗肝损伤作用。结果与模型对照组比较,正丁醇部位可明显降低CCl4引起的ALT、AST、MDA升高,增加肝组织中SOD的活性和GSH-Px的水平;70%乙醇洗脱的正丁醇提取物可明显降低CCl4引起的ALT、AST、MDA升高,增加肝组织中SOD的活性和GSH-Px的水平,且洗脱液剂量与保肝作用有明显的正相关性。结论太白楤木保肝作用的药效活性物质大多存在于70%乙醇洗脱的正丁醇部位,其抗肝损伤作用的活性成分可能与该部位主要成分皂苷类有关。
Objective To study the pharmacodynamic material basis of Aralia taibaiensis in anti - hepatic injury. Methods The experimental drugs were systematically extracted by solutions method （petroleum ether, chloroform, ethyl acetate, N - huoh） from Aralia taibaiensis according to solutions＇ polarity. The mice model of acute liver injury was established by carbon tetrachloride. Activities of ALT, AST, SOD were examined, and levels of MDA,GSH - Px were determined. The hepatic histological changes were observed by optical mi- croscope. The effect of anti - hepatic injury was compared. The effective part was in macroporous adsorption resin to purification, with dif- ferent concentration gradient itself on ethanol. Then the Same procedures were as above. And the effect of anti - hepatic injury was com- pared in each elution part. Results Compared with model control group, N - buoh extract of Aralia taibaiensis could significantly de-crease the ALT,AST,MDA activities by carbon tetrachloride, improve SOD activity and GSH -Px level. Pathological results showed that N - buoh extract of Aralia taibaiensis could significantly reduce the liver injury induced by carbon tetrachloride. The elution things by 70% ethanol from N - buoh extract could significantly decrease the ALT,AST,MDA activities by carbon tetrachloride, improve SOD activ- ity and GSH - Px level. Dosages of eluent were positively related to anti - hepatic injury. Conclusion The effective parts for anti - hepat- ic injury exist mostly at the part of 70% ethanol from N - buoh extract. The active ingredients of anti - hepatic injury might be combined with it＇s saponins ingredients.
出处
《医学研究杂志》
2012年第10期69-72,共4页
Journal of Medical Research
基金
陕西省教育厅资助项目(09JS015
2010JK506)
陕西省中管局资助项目(ZY30
ZY31)
关键词
太白楤木
抗肝损伤
有效部位
Aralia taibaiensis
Anti - hepatic injury
Effective composition
