摘要
目的 了解乙型肝炎病毒 (HBV)前C区基因突变与HBeAg阴性的原发性肝癌患者HBV感染的关系。方法 应用套式PCR方法对广西 16例原发性肝癌患者血清HBVDNA前C区进行扩增 ,对阳性者用SangerDNA测序法分析。结果 16例肝癌患者中有 14例HBVDNA阳性 ,阳性率为87 5 % (14/16 )。其中 1例HBeAg阳性 ,1例虽然HBeAg为阴性 ,但其前C区序列正常 ;2例为HBV野毒株和突变株混合感染 ;其余 8例为HBV突变株感染。该 8例中 ,仅第 4、5、12例的第 2 8密码为终止密码 ,有 1例为HBV终止密码株和无终止密码突变株混合感染。结论 经典的 1896位核苷酸点突变株感染在广西原发性肝癌患者中并不常见 ,提示可能存在前C区以外的基因突变 ,并导致HBeAg阴性的HBV感染。
Objective To study the association between mutation of hepatitis B virus (HBV) pre C gene and HBV infection in patients with primary hepatocellular carcinoma (PHC) but negative hepatitis B e antigen (HBeAg) in Guangxi Province, China. Methods Nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA Pre C region in sera collected from 16 patients with PHC in Guangxi, and then their HBV DNA nPCR products were sequenced by Sanger method. Results Sera in fourteen of 16 patients showed positive HBV DNA, with a positive proportion of 87.5% (14/16). One (C23) of them was positive for HBeAg; one (C24) was negative HBeAg, but with normal sequence in his Pre C region; two (C7, C14) were co infected with HBV wild and mutant strains; and the remainder eight cases (C3、 C4、 C5、 C8、 C10、 C11、 C12、 C13) were infected with mutant virus strains, but stop codon at codon 28 was only found in C4, C5 and C12. One case (C8) was co infected with both HBV stop codon mutant and non stop codon strains. Conclusion It is uncommon for patients with PHC in Guangxi infected with HBV mutant strain with classical mutation at nt 1 896, which suggests that maybe there exist other types of mutation other than that in pre C region causing HBV infection without HBeAg.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2000年第2期83-85,共3页
Chinese Journal of Preventive Medicine
基金
卫生部科研基金!( 98 1 3 5 1)
关键词
乙型肝炎病毒
基因突变
肝肿瘤
Hepatitis B virus
Genes
Mutation
Liver neoplasms
