摘要
目的 :探讨 p16、Rb、cyclinD1和EGFR在星形细胞肿瘤发生、发展中的作用及其与预后的关系。 方法 :免疫组化染色采用即用型S P法 ,用图像分析系统对 80例星形细胞肿瘤瘤细胞进行DNA含量测定。结果 :80例星形细胞肿瘤 :p16、Rb、cyclinD1和EGFR的阳性率分别为 43 8%、71 3%、5 5 0 %和 85 0 % ,4种蛋白阳性率及DNA含量与组织学分级均有统计学意义 ;p16、Rb、cyclinD1阳性率和瘤细胞DNA含量与预后有关 ,EGFR阳性率与预后无关 ;Rb失表达时 ,p16表达增强。 结论 :p16和Rb在抑制星形细胞肿瘤的生长及EGFR和cyclinD1促使其生长中起着重要作用 ,p16表达同DNA含量相结合评估星形细胞瘤预后有一定价值。
Purpose To study the DNA contents and the expressions of p16, Rb, cycli2n D1 and EGFR in the human astrocytic tumors. Methods Immunohistochemical study by S P kit. In order to analyze DNA contents of tumor cells, the tissue slices were stained by Feulgen method and analyzed using CMIAS Ⅱ in 80 cases astrocytic tumors. Results ①The positivity rates of p16, Rb, cyclin D1 and EGFR in the 80 cases of astrocytic tumors were 43 8%, 71 3%, 55 0% and 85 0% respectively; the DNA content and positivity rates were correlated to the pathological grading of these tumors. ②The DNA content and the expressions of p16, Rb and cyclin D1 were correlated to the patient survival rate, but EGFR was not. ③ Both p16 and Rb could be simultaneously detected in 13 of 80 case. p16 expression could be intensive in some cases that Rb were not expression. Conclusion The abnormal expression of p16, Rb, cyclin D1 and EGFR may play an important role in the pathogenesis of human astrocytic tumors. Both the DNA content and expression of p16 might be used as a valuable marker to determine the prognosis of this tumor.
出处
《临床与实验病理学杂志》
CAS
CSCD
2000年第1期44-47,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
脑肿瘤
星形细胞瘤
癌基因蛋白
DNA含量
brain neoplasms
astrocytoma
oncoprotein
p^(16) protein
prognosis
immunohistochemistry
