摘要
目的提高苦瓜中总黄酮提取效率,筛选出其最佳提取工艺。方法以苦瓜为原料,选取非离子型表面活性剂用于协同提取苦瓜中的总黄酮,研究表面活性剂用量、提取温度、提取时间、乙醇体积分数等因素对其影响。采用L9(34)正交试验确定苦瓜总黄酮的最佳提取工艺条件。结果 Tween-20对苦瓜中总黄酮的提取具有较好的协同作用。各因素对黄酮提取率影响大小依次是:表面活性剂用量>提取温度>乙醇体积分数>提取时间。结论最佳提取工艺条件乙醇浓度70%,Tween-20用量0.05g,提取时间4h,提取温度70℃。
Objective To improve the extracting efficiency of total flavonoids from bitter melon,and filter out the best extracting process.Methods Experiments were carried out to explore the extraction of flavonoids from the bitter melon.The effects of surfactant usage,extracting temperature,extracting duration,ethanol volume percentage on extraction effect of total flavonoids from bitter melon were discussed.L9（34）orthogonal experiment was used to obtain the optimum extraction conditions of total flavonoids.Results Tween-20 was suit to explore the extraction of flavonoids from the bitter melon.Various factors on extraction effect sizes was as following：surfactant usageextraction temperature ethanol volume percentageextracting duration.Conclusion The optimum extraction conditions were obtained as ethanol concentration 70%,Tween-20 0.05g,extraction at 70℃for 4 hours.
出处
《济宁医学院学报》
2012年第5期325-327,343,共4页
Journal of Jining Medical University
关键词
表面活性剂
黄酮
苦瓜
正交试验
Surfactant
Flavonoids
Bitter melon
Orthogonal experiment
