摘要
为了解多浪羊发情周期各阶段阴道细胞类型的变化情况,试验利用阴道涂片法对5只发情周期正常的多浪羊进行连续20 d的阴道涂片和镜检观察。结果表明:阴道细胞主要由角质化细胞、有核上皮细胞和白细胞组成。发情期角质化细胞占多数,发情后期3种细胞类型数量相近且差异不显著(P>0.05),发情间期主要以白细胞为主,发情前期有核上皮细胞占多数。根据细胞类型的变化确定发情周期,不同阶段的持续时间分别为发情后期(4±1)d、发情间期(9±3)d、发情前期(16±3)d、发情期(2±1)d。说明阴道细胞涂片法能较准确地确定绵羊发情周期的不同阶段。
To ascertain the changes of vaginal cells at different stages of estrous cycle in Duolang sheep, vaginal smear method was used in five Duolang sheep with a normal estrous cycle, and the vaginal smear samples were collected and continuously observed by microscopic examination for 20 days. The results showed that the vaginal cells were mainly composed of keratinocytes, nucleated epithelial cells, and leukocytes. Kerati- nocytes constituted the majority in estrus, and there were no significant differences （P 〉 0.05 ） among three kinds of cells with similar number in metestrus ; most of the cells were leukocytes in diestrus ; most of the ceils were nucleated epithelium cells in proestrus. According to the chan- ges in cell types, the estrous cycle was determined , it was found that the duration of the different stages was as follows ： metestrus （4 ± 1 ） d, diestrus （9 ± 3 ）d, proestrus （ 16 ±3 ）d, and estrus （2± 1 ）d. It is indicated that the vaginal smear method can accurately determine the differ- ent stages of estrous cycle in Duolang sheep.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2012年第11期35-37,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
"973"前期专项项目(2009CB125907)
国家自然科学基金项目(30960249)
关键词
绵羊
多浪羊
阴道涂片
发情周期
细胞类型
sheep
Duolang sheep
vaginal smear
estrous cycle
vaginal cells
