摘要
目的:探讨胰岛素对高糖环境下大鼠颌骨骨髓基质细胞成骨分化过程中prohibitin表达的影响。方法 :从Wistar大鼠下颌骨中分离培养骨髓基质细胞,分别于不同糖浓度(5.5、25、45 mM)及胰岛素(0、10-5M)的干预下成骨诱导(5.5 mM组、5.5 mM+Ins组、25 mM组、25 mM+Ins组、45 mM组、45 mM+Ins组)。成骨诱导21d,以茜素红染色进行矿化结节定量分析。成骨诱导1、3、7、14、21 d,ELISA试剂盒检测prohibitin蛋白的表达。采用SPSS15.0软件包对数据进行统计学分析。结果:45 mM高糖环境显著抑制了rBMSC的矿化,胰岛素可改善高糖对rBMSC矿化的抑制。45 mM组prohibitin的表达较5.5 mM组和25 mM组均显著下降。矿化诱导第3天,各组prohibitin蛋白的表达均达到最高峰。在25 mM和45 mM的糖浓度下,10-5M的胰岛素可显著上调rBMSC prohibitin蛋白的表达。结论:高糖环境抑制prohibitin蛋白的表达,胰岛素可改善高糖环境对prohibitin蛋白在骨髓基质细胞成骨分化过程中表达的影响。prohibitin蛋白在骨髓基质细胞成骨分化早期表达显著。
PURPOSE： To explore the effect of insulin on the expression of prohibitin in osteogenic differentiation of rat ＇s mandibular bone marrow stromal cells（BMSC） in high glucose.METHODS： Bone marrow stromal cells were obtained from the mandible of Wistar rats and stimulated in three glucose concentrations mineral medium（5.5,25,45 mM） with or without insulin（10-5 M） for 1,3,7,14 and 21 days.The expression of prohibitin was quantified via enzyme-linked immunosorbent assays（ELISA）.The mineralization nodules assessment was performed at day 21 by alizarine red staining. The statistics analysis was performed using SPSS 15.0 software package.RESULTS： High glucose of 45 mM inhibited mineralization of rBMSC and insulin could improve the mineralization in high glucose.The expression of prohibitin of 45 mM group decreased significantly compared with 5.5 mM group and 25 mM group.The expression of prohibitin of each group achieved maximum at day 3.Insulin（10-5 M） increased the expression of prohibitin of 25 mM group and 45 mM group in osteogenic differentiation of rBMSC.CONCLUSIONS： High glucose inhibited the expression of prohibitin in osteogenic differentiation of rBMSC,and insulin can improve the effect.Prohibitin may play an important part in early stage in osteogenic differentiation of rBMSC.
出处
《上海口腔医学》
CAS
CSCD
北大核心
2012年第5期526-530,共5页
Shanghai Journal of Stomatology
