摘要
目的:研究模拟微重力(SMG)对人牙髓干细胞(hDPSCs)在聚乳酸-羟基乙酸(PLGA)支架上矿化的影响。方法:采用酶消化法分离、培养人牙髓干细胞,并进行鉴定。hDPSCs常规接种于PLGA支架,24h后,模拟微重力环境和普通环境下分别矿化诱导。矿化诱导第3、5、7、10、14天时进行碱性磷酸酶活性检测,第21天时进行茜素红染色以观察人牙髓干细胞在PLGA支架上矿化结节形成情况。结果:模拟微重力环境下的碱性磷酸酶活性明显低于普通环境(P<0.01),茜素红染色显示模拟微重力环境下形成的矿化结节较普通环境下的小且数量少。结论:模拟微重力环境抑制hDPSCs在PLGA支架上的矿化。
Objective： To investigate the effect of Simulated Microgravity on mineralization of human dental pulp stem cells （hDPSCs） on PLGA （Poly lactic acid glycolic acid） scaffolds. Methods： The hDPSCs were inoculated on PLGA scaffolds for 24h after routine isolation, culture and identification, then cultured in simulated microgravity environment or conventional mineralization-induced environment, respectively. Alkaline phosphatase testing by 3, 5, 7, 10, 14 days and Alizarin red staining by 21 days were detected. Results： ALPase testing demonstrated lower ALPase activity in simulated microgravity environment at various time points compared with the control group. A- lizarin red staining showed positive granules were hardly detected in simulated microgravity group, however, noted in the control group. Conclusion： Together, these results indicate that simulated microgravity inhibits mineralization of hDPSCs on PLGA scaffolds.
出处
《口腔医学研究》
CAS
CSCD
2013年第2期135-137,共3页
Journal of Oral Science Research
基金
国家自然科学基金(项目批准号:81271132)
关键词
人牙髓干细胞
模拟微重力
矿化
Human dental pulp stem cells Simulated microgravity Mineralization
