摘要
目的探讨癌痛消方中使用的大剂量活血化淤药物对肝癌细胞凋亡因子的影响,进一步明确其在抗肿瘤方面的作用机制。方法将40只Walker-256移植性肝癌大鼠模型随机分为4组,即癌痛消全方组、活血化淤药组、非活血化淤药组及模型组,于造模后第8天给药,连续用药14 d后停药24 h,处死大鼠,并取出适量肿瘤组织行流式细胞JC-1染色法检测,并取出适量瘤块行免疫组化法检测其中Fas、FasL、Caspase-3蛋白的表达情况。结果肝癌细胞线粒体膜电位下降的细胞比值(JC-1+%)在活血化淤药组中为(22.34±1.94)%,与癌痛消全方组、非活血化淤组两两比较,差异均显著(P=0.000),与模型组比较,差异无统计学意义(P=0.093)。Fas、FasL和Caspase-3蛋白在活血化淤药组的表达情况与模型组比较,差异无统计学意义(P=0.835、P=0.584和P=0.820);与癌痛消全方组和非活血化淤药组两两比较,差异均有统计学意义(P<0.01或P<0.05)。三者蛋白表达情况与细胞凋亡检测结果相一致。结论单独使用癌痛消方中大剂量活血化淤药不能明显影响Fas、FasL和Caspase-3蛋白在肝癌细胞中的表达,不能明显干扰细胞凋亡网络信号从而促进肝癌细胞的凋亡,有必要与其他具有抗肿瘤作用药物联合使用。
Objective To clarify the role of blood circulation drugs used heavily in ATXP in anti - tumor mechanism, by exploring its impact on hepatocellular carcinoma cell apoptotic regulators. Methods 40 rats with walker - 256 cell lines transplanted hepato- ma were randomly divided into four groups, namely, ATXP group, blood circulation drugs group, non - blood circulation drugs group and model group. The drugs were given on the eighth day after the successful modeling continuously for 14 days, then the rats were sacrificed 24 hours later, and the tumor tissue was taken out for flow cytometry of JC - 1 staining and immunohistochemical assay of protein expression of Fas, FasL and Caspase - 3. Results The ratio （ JC - 1 ＋ % ） of HCC cell with decrease of mitochondrial membrane potential was （ 22.34 ± 1.94） % in the blood circulation drugs group, compared with the ATXP group and the non - blood circulation group, the differences were statistically significant （ P = 0.000）. But compared with model group, the difference was not statistically significant （ P = 0. 093 ）. Fas, FasL and Caspase - 3 protein expression in the blood circulation drugs group compared with model group,the differences were not statistically significant （P = 0. 835 ,P = 0. 584 and P = 0. 820） ; however compared with the ATXP group the non- blood circulation group, the differences were statistically significant （P 〈 0.01 or P 〈 O. 05 ）. The results of flow cytometry and immunohistochemieal assay were consistent. Conclusion Large doses of blood circulation drugs from ATXP alone cannot significantly affect the expression of Fas, FasL and Caspase -3 protein in hepatoma cells, and can not significantly interfere with the apoptosis network signal to promote the apoptosis of hepatoma cells. Therefore, it is necessary to use the blood circulation drugs in combination with other anti - tumor drug.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第10期2401-2403,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30960475)
