摘要
测定了小叶锦鸡儿(Caragana microphylla Lam.)花粗提物和不同极性组分总黄酮含量和各项抗氧化能力指标。结果表明:小叶锦鸡儿花不同极性的黄酮组分的抗氧化活性有明显差别,其总抗氧化能力与溶剂极性之间有显著正相关性(P<0.05),但其它抗氧化活性与溶剂极性之间均无显著相关性(P>0.05)。同一极性黄酮组分在不同抗氧化体系中的抗氧化活性有较大差别。对DPPH.自由基的清除能力最强的为乙酸乙酯层(EC50=0.11 mg/mL),其次是二氯甲烷层(EC50=0.38 mg/mL)和乙醇粗提物(EC50=0.56 mg/mL)。总抗氧化能力(T-AOC)最强的为水层,在1 mg/mL时为1.482个T-AOC能力单位。清除羟自由基.OH能力最强的为水层,其次为正己烷层和乙醇粗提物,在3.57 mg/mL时清除率分别为86.23%、66.04%和64.63%。清除超氧阴离子自由基O2-.能力最强的为水层,其次为乙醇粗提物,在1 mg/mL时其清除率分别相当于0.15 mg/mL Vc清除率的336.17%和318.20%。可见,小叶锦鸡儿花的粗提物和不同极性黄酮组分具有较好的抗氧化活性,可用于提取、制备抗氧化特性和作用不同的天然抗氧化剂。
The total flavonoid content and antioxidative activities of crude extract and flavonoid fractions of C. microphylla flowers with different polarity were investigated. The results showed that the antioxidative activity of flavonoid fractions varied markedly with their polarity. There was a significant positive correlation between total antioxidative capacity （TAOC） and the polarity of the flavonoid fractions （ P 〈 0.05 ） but no significant correlations were found between scavenging activities of DPPH-, hydroxyl and superoxide anion radicals and the polarity of the flavonoid fractions （ P 〉 O. 05 ）. Moreover, the antioxidative activities of the same fraction varied in different antioxidative determination systems.Ethyl acetate fraction exhibited the highest DPPH · scavenging activity （EC50= 0. 11 mg/mL）,foUowed by dichloromethane fraction （ EC50=0.38 mg/mL） and crude extract （ EC50= 0.56 mg/mL）. H20 fraction showed the highestT-AOC, with 1. 482 units of T-AOC at 1 mg/mL. H20 fraction showed the highest hydroxyl free radical scavenging activity,followed by n-hexane fraction and crude extract,with scavenging rates of 86.23%, 65.04% and 64.63% at 3.57 mg/mL,respectively. H20 fraction displayed the highest superoxide anion radical scavenging activity,followed by ethanolic crude extract,with scavenging rates of 336.17% and 318.20% at 1 mg/mL relative to that of 0. 15 mg/mL Vc, respectively. These data suggested that extract and flavonoid fractions of C. microphylla flowers had strong antioxidative activity, and can be potential source for natural antioxidants of different antioxidative properties and applications.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2012年第10期1348-1354,共7页
Natural Product Research and Development
基金
This work was co-financed by Research Project Supported by Shanxi Scholarship Council of China(2012-109)
PhD Awardees Research Foundation,Shanxi Academy of Agricultural Sciences(YBSJJ1108)
关键词
小叶锦鸡儿
花
极性
黄酮类化合物
抗氧化活性
Caragana microphylla Lam.
flowers
polarity
flavonoids
antioxidative activity
