摘要
目的观察大黄酸对糖尿病大鼠肾脏氧化应激的影响。方法链脲佐菌素(STZ)诱导的糖尿病肾病大鼠模型随机分为模型组与治疗组,治疗6周后,比较各组大鼠肾组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,同时检测各组大鼠血糖、血肌酐、尿素氮、24 h尿蛋白定量等。采用逆转录-聚合酶链反应(RT-PCR)方法检测p22phox及p47phox的表达。结果治疗组较模型组明显改善尿清蛋白、尿素氮水平和肾脏肥大指数。肾脏MDA含量明显下降(P<0.05),SOD活性显著上升(P<0.05)。与对照组比较,模型组p47phox和p22phoxmRNA表达明显增多,大黄酸干预使其表达明显降低。结论大黄酸对2型糖尿病肾脏病变有一定的保护作用,其机制可能是通过抑制氧化应激反应,下调糖尿病大鼠p22phox及p47phox的表达对2型糖尿病模型大鼠肾脏产生保护作用。
Objective To observe the effect of Rhein on oxidative stress of kidney in diabetic rats.Methods Streptozotocin（STZ）-induced diabetic nephropathy rats were randomly divided into model group and treatment group.Malondialdehyde（MDA） content and superoxide dismutase（SOD） activity in the rat renal tissues of the two groups were compared six weeks after the treatment.At the same time,blood glucose,serum creatinine,blood urea nitrogen,and 24 h urine protein quantitation were detected.Expression of p22phox and p47phox was determined by RT-PCR.Results Urinary albumin,urea nitrogen level and kidney hypertrophy index were significantly improved in the treatment group,as compared with the model group.Kidney MDA content was decreased significantly（P〈0.05）,and the activity of SOD increased significantly（P〈0.05）.As compared with the control group,mRNA expression levels of p47phox and p22phox in the model group were increased significantly,and the expression levels were significantly reduced after Rhein intervention.Conclusion Rhein plays a protective role in type 2 diabetic nephropathy probably through inhibition of oxidative stress in the diabetic rats.Down-regulating p22phox and p47phox expression in type 2 diabetic model rat kidney can protect rat kidney.
出处
《医药导报》
CAS
北大核心
2012年第10期1285-1288,共4页
Herald of Medicine
