摘要
目的 了解血管紧张素受体拮抗剂缬沙坦对 2型糖尿病大鼠肾脏病变的保护作用及其机制。 方法 高糖、高脂饮食加小剂量链脲佐菌素 (STZ) 30mg/kg制作 2型糖尿病动物模型 ,将实验动物分为正常对照组、糖尿病组及缬沙坦治疗组 ,检测各组大鼠的血糖、血胰岛素、血肌酐、尿素氮、尿白蛋白、肾脏肥大指数的变化 ,免疫组化检测PAI 1的蛋白表达水平。 结果 缬沙坦治疗组较非治疗组尿白蛋白明显减少 (P <0 .0 1) ,尿素氮水平下降 (P <0 .0 5 ) ,肾脏肥大指数改善 (P <0 .0 5 ) ,免疫组化显示 ,糖尿病组大鼠肾小球PAI 1蛋白表达增多 ,治疗组PAI 1蛋白表达明显降低。 结论 缬沙坦对 2型糖尿病肾脏病变有一定的保护作用 ,其机制可能是通过下调糖尿病大鼠PAI
Objective To investigate the mechanism of valsartan in protecting the kidney in type 2 diabetic rats. Methods The fat fed ,strepozotocin treated rats were served as experimental animal model. Adult female Sprague Dawley rats ( n =10 in each group) were studied:normal control rats,diabetic rats and diabetic rats treated with valsartan (10 mg· -1 ·d -1 , Plasma glucose,serum insulin,serum creatinine,urinary albumin,BUN and profile of kidney hypertrophy were observed after 10 weeks of treatment. PAI 1 protein expression was measured by immunohistochemistry. Results Urinary albumin excretin( P< 0.01)、BUN( P <0.05)and kidney hypertrophy index( P <0.05)of valsartan treated group were significantly lower than those of diabetic group. The protein expression of PAI 1 in the valsartan group was lower than that in the diabetic group. Conclusion Valsartan may has kidney protective effect on diabetic rats,partly through down regulated PAI 1expression
出处
《中国糖尿病杂志》
CAS
CSCD
2003年第5期337-340,共4页
Chinese Journal of Diabetes
