摘要
能源危机引发人们对可再生生物能源的广泛关注。本文以潜在能源栅藻为对象,采用不同pH、不同M试剂浓度的改良BG11培养基对其培养条件进行优化,并利用超声波提取、振荡提取和索氏提取法结合不同试剂对其脂质提取效率进行比较研究。结果表明,栅藻在偏碱性环境中生长较好,在改良BG11培养基中培养30d,其生物量、脂质总量和脂质产率分别为对照组(普通BG11培养基)的7.8倍、3.8倍和3.9倍;栅藻经真空冷冻干燥后,用石油醚超声提取,细胞内脂质提取效率最高(脂质含量为27%),与常规的氯仿/甲醇法相比,其得到的脂质含量提高8%。进一步研究证实,脂质提取效率相对较高的4种方法均采用超声波提取,表明超声波提取是一种行之有效的脂质提取方法。
Biofuels draw increasing public attention because of the energy crisis.In this study,the effect of pH and M reagent on growth of Scenedesmus sp.was studied and the lipid extraction efficiency of various methods,including ultrasound extraction,shaking extraction and Soxhlet extraction was compared.The results showed that Scenedesmus sp.grew better under alkaline conditions and its growth condition and lipid production could be significantly improved in the modified BG 11 medium.After 30d,Scenedesmus sp.in modified BG 11 medium performed a higher production of biomass,lipid and lipid productivity,which was 7.8 times,3.8 times and 3.9 times of that of the control group.It was also shown that the ultrasound-assisted petroleum ether extraction coupled with vacuum freeze-drying was identified as the optimum lipid extraction method among the methods tested,in which the lipid content was 27% which was about 8% higher than that when using the chloroform-methanol extraction method.Further research confirmed that ultrasound extraction was the most efficient one among all the methods.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第6期894-899,共6页
Journal of Nuclear Agricultural Sciences
基金
农业部公益性行业科研专项-核技术农业应用(201103007)
国家自然科学基金面上项目(20977076)
关键词
栅藻
生物柴油
培养条件优化
脂质提取
Scenedesmus sp.
biodiesel
medium modification
optimization of lipid extraction
