摘要
利用实验室中筛选到的假单胞菌P1-1菌株进行脱氮除磷研究,并探讨了温度、pH、接种量、摇床转速和碳源对该菌株生长和脱氮除磷的影响,同时研究了菌株除磷的动力学。结果表明,该菌株最佳生长条件为摇床转速140 r/min、接种量10%、pH 8.0、温度30℃,最佳除磷条件为摇床转速140 r/min、接种量10%、温度30℃、pH 8.0,最佳脱氮条件为接种量10%、摇床转速0、温度30℃、pH 7.0;该菌株在比较好的生长和脱氮除磷效果时需要乙酸钠的最低浓度为1 000 mg/L;当初始P浓度为6~21 mg/L时,菌株除磷的动力学过程符合Monod零级反应模型。
Pseudomonas sp.Strain P1-1 which was screened in laboratory was studied for its nitrogen and phosphorus removal characteristics.Effects of temperature,pH,bacteria amount,shaking speed and carbon source on growth as well as nitrogen and phosphorus removal of the strain were discussed.Kinetics of the strain in the phosphorus removal was also studied.Results indicated that the optimum conditions for the growth of the strain were shaking speed at 140 r/min,inoculation 10%,pH 8.0 and temperature at 30 ℃,while the optimum conditions for phosphorus removal by the strain were shaking speed at 140 r/min,inoculation 10%,temperature at 30 ℃ and pH 8.0,and the optimum conditions for nitrogen removal by the strain were inoculation 10%,shaking speed at 0 r/min,temperature at 30 ℃ and pH 7.0.The lowest concentration of sodium acetate for the good growth and nitration and phosphorus removal of the strain was 1 000 mg/L.The kinetics of phosphorus removal between 6 mg/L and 21 mg/L were preliminary studied with the Monod model zero-order reaction.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2012年第10期29-33,共5页
Environmental Science & Technology
基金
国家自然科学基金项目(50874032
41073060)
上海市重点学科建设项目(B604)
关键词
反硝化聚磷菌
正交实验
动力学
denitrifying phosphate-accumulating organisms（DNPAOs）
orthogonal test
kinetics
