摘要
高通量测序技术是DNA测序发展历程的一个里程碑,它为现代生命科学研究提供了前所未有的机遇。详细介绍了以454、Solexa和SOLiD为代表的第二代高通量测序技术,以HeliScopeTIRM和Pacific Biosciences SMRT为代表的单分子测序技术,以及最近Life Science公司推出的Ion Personal Genome Machine(PGM)测序技术等高通量测序技术的最新进展。在此基础上,阐述了高通量测序技术在基因组测序、转录组测序、基因表达调控、转录因子结合位点的检测以及甲基化等研究领域的应用。最后,讨论了高通量测序技术在成本和后续数据分析等方面存在的问题及其未来的发展前景。
As a milestone in the development of DNA sequencing,high-throughput sequencing technology provides an unprecedented opportunity for the modern life sciences.The recent progress on this technology,including the second generation sequencing technology（represented by 454,Solexa and SOLiD）,the third generation sequencing technology（represented by HeliScope TIRM and Pacific Biosciences SMART）and the Ion Personal Genome Machine sequencing technology are summarized.Then,the application of the high-throughput sequencing technology in genome sequencing,transcriptome sequencing,gene expression regulation,detection of binding locations for transcription factors and methylation analysis are summarized.Finally,the disadvantages and the prospects of this technology were discussed.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2012年第1期109-114,共6页
China Biotechnology
基金
山西省青年科技研究基金(2010021030-1)
国家自然科学基金(31100235)资助项目
关键词
高通量测序
深度测序
下一代测序
基因组测序
转录组测序
High-throughput sequencing Deep sequencing Next generation sequencing Genome Sequence Transcriptome sequence
