摘要
[ Objective] This paper aimed to find out the relationship between pathogenic procine E. coli virulence gene and pathogenicity, and ex- plore the pathogenic mechanism of E. coil [ Methed] The detection of two E. coil virulence genes was performed. PCR method was taken to test the virulence genes, astA and stb, from 39 strains of typical serotype O porcine E. coli which had been separated and identified. [ Result] It＇s found that of the 39 isolates of porcine E. coli, 22 carried virulence gene astA which represented 56.41%, and 27 carried virulence gene stb which repre- sented 69.23%. [ Conclusion] This study has provided scientific data for future E. coil pathogenicity researches.
[ Objective] This paper aimed to find out the relationship between pathogenic procine E. coli virulence gene and pathogenicity, and ex- plore the pathogenic mechanism of E. coil [ Methed] The detection of two E. coil virulence genes was performed. PCR method was taken to test the virulence genes, astA and stb, from 39 strains of typical serotype O porcine E. coli which had been separated and identified. [ Result] It＇s found that of the 39 isolates of porcine E. coli, 22 carried virulence gene astA which represented 56.41%, and 27 carried virulence gene stb which repre- sented 69.23%. [ Conclusion] This study has provided scientific data for future E. coil pathogenicity researches.
基金
Science Technology department Project of Hebei Province(NO.08220401D,NO.10960408D)
Forty Seven of China Postdoctorate Surface Science Fund(NO.20100470565)
Science Technology department Project of Qinhuangdao (NO.201101A182)
