摘要
目的:研究抗苗勒管激素(AMH)及抗苗勒管激素Ⅱ型受体(AMHRⅡ)基因多态性在多囊卵巢综合征(PCOS)患者的表达情况;同时分析PCOS不同AMH基因型患者体重指数(BMI)、性激素水平之间是否存在差异,从基因水平探讨PCOS的发病机制。方法:采用聚合酶链反应(PCR)及DNA正、反向测序方法,检测94例PCOS患者(PCOS组)及94例正常排卵患者(对照组)AMH和AMHRⅡ基因型,同时将两组AMH、AMHRⅡ不同基因型患者的年龄、BMI及性激素水平分别进行比较。结果:①两组AMH基因型分布比较,差异有统计学意义(P<0.05),而AMH等位基因频率、AMHRⅡ基因型分布及等位基因频率分布比较,差异均无统计学意义(P>0.05)。②在PCOS组不同AMH基因型患者中,BMI、LH、T间差异有统计学意义(P<0.05),基因型分布类型与BMI、LH、T有关(P<0.05),而与年龄、FSH、E2、PRL无关(P>0.05);对照组年龄、BMI、性激素水平与AMH基因型分布无关(P>0.05)。③对照组和PCOS组年龄、BMI、性激素水平与AMHRⅡ基因型分布无关(P>0.05)。结论:AMH基因的多态性可能与PCOS的发病相关;PCOS患者AMH基因型中G/T表型可能是引起患者肥胖、高雄激素血症的原因。
Objective：To investigate the gene polymorphism of the anti,mullerian hormone （AMH） and its type Ⅱ receptor （AMHR Ⅱ ） in polycystic ovarian syndrome （ PCOS）, and to analyze the relationship of body mass index （BMI） and sex hormone levels in patients with different AMH gene types, so as to explore the possible mechanism of PCOS in gene level. Methods： Genotype distribution and allele frequencies of AMH and AMHR Ⅱ were examined by polymerase chain reaction （PCR） and DNA forward and reverse se- quencing in 94 PCOS patients and 94 normal women as control. The age, BMI and hormone levels of differ- ent AMH, AMHR Ⅱ genotypes were compared. Results：There was a significant difference of AMH geno- type distribution in the two groups （P 〈0.05） ,while the AMH allele frequencies, AMHR I] gene distributions and its allele frequency distribution did not show marked difference in two groups （P 〉 0.05） ; ~）There were obvious difference of BMI, LH, T levels in PCOS patients with different AMH genotypes （ P 〈 0.05）. The genotype distribution was correlated with BMI,LH and T levels（P〈0.05） but not correlated with age, FSH, E2 and PRL（ P〉O. 05）. In the control group, there was no correlation between AMH genotype distribution and patient age, BMI and sex hormone levels（ P 〉 O. 05）.There were no correlations between the AMHR2 genofype distribution and patient age, BMI and sex hormone levels in either normal control or PCOS patients （~ P 〉0.05）. Conclusions：AMH gene polymorphism may be associated with the pathogenesis of PCOS, al- so the G/T phenotype in AMH genotype may be the reason for obesity and hyperandrogenism in PCOS.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2012年第9期739-742,共4页
Journal of Practical Obstetrics and Gynecology
基金
国家自然科学基金-青年基金课题(编号:81100421)
宁夏自然科学基金资助项目(编号:NZ10115)
宁夏自治区科技攻关项目(编号:KF2010-49)
