摘要
以胞苷一磷酸(CMP)为底物,采用响应面分析法对固定化酵母细胞发酵制备胞苷三磷酸(CTP)的影响因素进行优化,采用HPLC法对CTP定量。结果表明:在CTP生物合成中磷酸根离子和Mg2+浓度存在一个临界浓度低于临界浓度二者将限制CTP产率;在本实验范围内固定化酵母发酵制备CTP的最佳工艺为:20mL底物样液添加20g固定化酵母,CMP浓度100mmol/L、葡萄糖浓度100mmol/L、磷酸盐浓度250mmol/L、Mg2+浓度15mmol/L、pH6.1、反应时间2h,在此条件下CTP的产率可达67.89%。
Response surface methodology was employed to optimize the biosynthesis of cytidine triphosphate （CTP） from cytidine monophosphate （CMP） by immobilized yeast cells. CTP was analyzed by HPLC. Critical phosphate ion and Mg^2＋ concentrations for CTP synthesis were found and CTP yield was restricted when phosphate ion and Mg^2＋ were lower than their respective critical concentrations. The optimal CTP synthesis conditions were determined as follows： 20 g of immobilized yeast cells were cultured in 20 mL of 100 mmol/L CMP containing 100 mmol/L glucose, 250 mmol/L phosphate and 15 mrnol/L Mg^2＋ at pH 6.1 for 2 h. Under these conditions, the yield of CTP was up to 67.89%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第19期259-263,共5页
Food Science
关键词
胞苷三磷酸
固定化酵母
生物转化
响应面分析法
cytidine tdphosphate
immobilized yeast
biosynthesis
response surface methodology
