摘要
对小蓟乙醇粗提物中显示细胞毒活性的石油醚萃取部位进行化学成分的研究。运用硅胶、凝胶和反相高效液相等色谱方法进行分离纯化;应用一维、二维NMR和MS等波谱学方法对纯化的物质进行结构鉴定。结果表明:从小蓟乙醇提取物的石油醚萃取部位分离鉴定了4个甾体类化合物,分别为:麦角甾-4,24(28)-二烯-3-酮(化合物1)、豆甾-4-烯-3-酮(化合物2)、豆甾醇(化合物3)和β-谷甾醇(化合物4)。化合物1和2为首次从蓟属中分离得到,除化合物4外,其余3个化合物均是首次从小蓟中分离得到;在体外MTT法的细胞毒活性测试中,所筛选的化合物在1×10-5mol/L的浓度条件下均未显示出细胞毒活性。
The petroleum ether-soluble fraction of pooled ethanol extracts from the aboveground parts of Cirsium setosum （Willd.） MB was further fractionated by silica gel column chromatography, Sephadex LH-20 column chromatography and preparative HPLC. The resulting sub-fractions were structurally identified by one-dimensional, two-dimensional NMR and MS spectroscopies. Four steroids were obtained, including ergosta-4, 24 （28）-diene-3-one （1）, stigmast-4-en-3-one （2）, stigmasterol （3） and fl-sitosterol （4）. To the best of our knowledge, this is the first report showing the presence of Compounds 1 and 2 in the genus Cirsium and the presence of Compounds 1, 2 and 3 in Cirsium setosum （Willd.） MB. None of these identified compounds showed cytotoxic activity at a concentration of 1 ×10^5 mol/L as determined by in vitro MTT assay.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第19期124-127,共4页
Food Science
基金
北京市教育委员会面上项目(KM201111417001)
关键词
小蓟
分离纯化
结构鉴定
甾体
细胞毒活性
Cirsiumsetosum
isolation andpurification
structural elucidation
steroids
cytotoxicity
