摘要
酶场效应晶体管(ENFET)是近年来发展起来的一类半导体技术和生物技术相结合的新型器件。双管差分输出式ENFET对环境温度变化及总体pH变化等外界因素有自动补偿作用,其输出电压随时间的漂移较之单管输出有很大的改善。此外,还可采用金电极作参比电极以实现探头的小型化。青霉素ENFET在0.01mol/L和0.02mol/L的磷酸缓冲液中的响应灵敏度分别为6.5—7.0mV/mmol/L和3.2—3.6mV/mmol/L,线性范围为0.5—14mmol/L和0.5—25mmol/L。当浸于0.01mol/L磷酸缓冲液、置于4℃下保存时,探头的贮存寿命大于6个月。探头的累计使用次数可超过1000次。本文还论述了青霉素ENFET在青霉素发酵液浓度测定中的应用。
Enzyme FET is a kind of new device developed recently, which is made through the combination of semiconductor and biological technology. Enzyme FET with differential output can provide automatic compensation for someexternal factors, e. g. variations of temperature and pH of bulk solution. By using differential measurement, drift of the output voltage with time is improved, and the sensor can be miniaturized through the use of 'pseudo-reference electrode'. Differertial model penicillin-ENFET was constructed by modifying one of the dual ISFET gate with a membrane of cross-linked bovin serum albumin (BSA)-penicillinase and the other with a membrane of cross-linked BSA. It responsed linearly to penicillin in 0.01mol/L and 0.02mol/L phosphate buffer with sensitivities of 6.5—7.0mV/mmol/L and 3.2—3.6mV/mmol/L over the concentration ranges of 0.5—14mmol/L and 0.5—25mmol/L, respectively. When immersed in 0.01mol/L phosphate buffer and stored in 4℃, this detector had a life time of 6 months with only a slight decline of output. Accumulative total usage times of the detector could be over one thousand. Finally, the Practical application of differential model penicillin-ENFET in assaying penicillin concentration of fermentation broth is concerned.
出处
《生物工程学报》
CAS
CSCD
北大核心
1990年第2期151-156,共6页
Chinese Journal of Biotechnology
基金
传感技术联合开放研究实验室资助课题
关键词
晶体管
酶场效应晶体管
青霉素
生物工程
H-ISFET
enzyme FET
immobilized enzyme membrane
assays of penicillin concentration
semiconductor chemical seneors
