摘要
为了检测化妆品中产生表皮剥脱性毒素的葡萄球菌，将护肤类化妆品中检出的革兰氏阳性球菌用普通营养 琼脂进行分纯培养，把分纯菌株制成氯化钠肉汤菌悬液，离心后取其上清液０．５ｍｌ注射至兔背部皮内，２４ｈ后观察其皮 肤反应，将引起皮肤烫伤综合征病变的菌株，再应用ＡＰＩ葡萄球菌鉴定试剂盒进行复核鉴定。结果发现除７株金黄色 葡萄球菌外，尚有松鼠、科氏、头状、溶血、腐生、解脲科氏、表皮、木糖葡萄球菌８种２３株革兰阳性球菌可引起皮 肤烫伤综合征病变。表明化妆品中除金黄色葡萄球菌可产生表皮剥脱性毒素外，其它少数凝固酶阴性葡萄球菌（ＣＮＳ）① 也可产生上述毒素。
Objective ]: To examine the exfoliative toxin of staphylococcus in cosmetics. [Methods ]: We cultured the different gram positive cocci in protecting skin cosmetics with normal nutrient agar, then making it to mix liquid, injecting 0. 5ml uper liquid into rabbit back after concentyifugation, examining the skin after 24h. The germ that can induce staphylococcal scalded skin syndrom determined by API staphylococcus diagnostic test. [Results ]: Showed that 8 kinds of staphylococcus, such as staphylococcus sciuri, staphylococcus cohnii capitis, haemolyticus, cohnii urealyticum saprophyticusepidermidis and xylosus, 23 strain gram w positive cocci induced staphylococcal scalded skin syndrom besides 7 staphylococcus aureus that we have recognized. [Conclutions]: It indicated some staphylococccus that coagulating enzymes are negative can also produce exfoliative toxin.
出处
《中国卫生检验杂志》
2000年第2期133-135,共3页
Chinese Journal of Health Laboratory Technology
基金
河北省科委规划课题!项目号982761122
河北省石家庄市卫生防疫站科研基金资助
