摘要
目的 采用培养、鉴定、菌落计数、药敏试验一体化检测的方法 ,分析支原体感染的机率与分布 ,并探讨其致病性与耐药性 ,为临床治疗提供可靠的实验依据。方法 我院妇科及皮肤科等 70 0例疑为NGU患者 ,女性取宫颈分泌物 ,男性主要取尿道分泌物 ,立即接种于专用培养基中 ,Uu在培养 2 4、48小时后各观察一次 ,Mh在培养48、72小时后各观察一次 ,详细记录结果。结果 70 0例标本中 ,发现支原体生长 2 0 0例 (菌落计数≥ 10 4 CFU ml) ,感染率为 2 8.5 %;其中解脲支原体 (Uu)感染 181例 (感染率为 90 .5 %) ,人型支原体 (Mh)感染 4例 (感染率为 2 %) ,Uu与Mh合并感染 15例 (敏感率为 7.5 %)。对此微生物的抗感染治疗 ,以交沙霉素为最佳 (敏感率 92 %) ,次为克拉霉素 (敏感率 89.5 %)与美满霉素 (敏感率 83 %) ,而壮观霉素 (敏感率 2 0 %)甲砜霉素 (敏感率 3 8%)和可乐必妥 (敏感率 3 8.5 %)的抗感染效果较差。结论 采用菌落计数法对支原体进行感染的致病性鉴别 ,符合临床诊断要求 ,可避免无科学实验依据的盲目诊断 ;采用药物定量稀释法对支原体进行药敏检测 ,追踪其耐药性 。
Objective To analyse the probability and distribution of mycoplasma infection and study their pathogenicity and drug resistance.In order to offer reliable information in clinical therapy.The culture,identificatian,bacterial counting and antimicrobial susceptibility test of mycoplasma were carried out with one complex mycoplasma kit.Methods Cervical secretion of female and urethral secretion of male were collected from 700 suspected NGU patients from gyrecological and dermatological clinic planted in special culture medium at once. Uu was observed after 24 hours and 48 hours,Mh was observed after 48 and 72 hours.Noted the results in detail.Results 200 mycoplasma was discovered in 700 specimens (bacterial counting≥10 4CFU/ml),infection rate was 28.5%.Ureaplasma urealyticum(Uu) was 181(90.5%),Mycoplasma hominis(Mh) was 4(2%) and mixed both infection was 15(7.5%).To cure this microorganisms,the best antibiotics is Josamycin(92%),the second is Clarithromycin(89.5%)and Minomycin(83%).Spectinomycine(20%)Thiamphenicol(38%)and Gravit(38.5%) are worse.Conclusions Using bacterial counting method to identify the pathogenicity of mycoplasmta infection is in according with clinical diagnose,can avoid blind diagnosis which has no scientific supporting. Drag sensitivity testing to mycoplasma by drug quantitative dilution method has an very important significance in directing how to use antibiotics in clinic.
出处
《海南医学》
CAS
2004年第9期16-17,23,共3页
Hainan Medical Journal
关键词
支原体
UU
MH
感染
致病性
耐药性
mycoplasma
Uu
Mh
infection
pathogenicity
drug resistance
