摘要
遗传多样性的量化与分类是收集和利用种质资源的重要前提。运用SSR和ISSR技术分析扁蓿豆种质资源的遗传多样性。从供试材料中筛选到具有多态性的SSR引物18对,ISSR引物18条。SSR引物共扩增到109条清晰的多态性条带,多态性比率为80.09%,相似系数变化范围为0.669～0.885。ISSR引物共扩增到125条清晰的多态性条带,多态性比率为87.08%,相似系数变化范围为0.448～0.811。对2种标记结果进行UPGMA聚类分析,结果表明,扁蓿豆材料部分聚为1类,黄花扁蓿豆材料大部分聚为1类。Mantel检测显示,2种标记的遗传相似性呈显著相关(r=0.019 6,t=0.121 2),扁蓿豆比黄花扁蓿豆的遗传多样性水平稍高,细叶扁蓿豆之间的遗传差异较大。
Quantitative and classification was based on germplasm collection and utilization.The genetic diversity of 14 wild Medicago ruthenica was assessed by screening with 18 pairs of SSR and 18 ISSR primers.SSR primers amplified 109 clear polymorphic bands,and the polymorphism rate was 80.09% with a similarity coefficient ranging from 0.669 to 0.885.ISSR primers amplified 125 polymorphic bands and the polymorphism ratio was 87.08% with a similarity coefficient ranging from 0.448 to 0.811.UPGMA（Unweighted Pair Group Method with Arithmetic mean） cluster analysis based on two markers showed that part of the M.ruthenica material grouped into one group.Mantel tests showed that the genetic similarity of two markers had a significant correlation（r=0.019 6,t=0.121 2）.Compared with yellow M.ruthenica genetic diversity levels,those of M.ruthenica were higher.The genetic difference was highest in M.ruthenica var.oblongifolia.
出处
《草业学报》
CSCD
北大核心
2012年第5期107-113,共7页
Acta Prataculturae Sinica
基金
农业部农作物种质资源保护与利用项目"多年生牧草更新复壮与利用"(NB2011-2130135-33)
农业部牧草种质资源保护项目"牧草温带备份库的维护与备份繁殖入库"(2010-43)资助
