摘要
目的 观察细胞骨架分子Fascin-1，Ezrin及Paxillin在喉鳞状细胞癌（简称鳞癌）组织中的表达，并评价三者对喉鳞癌复发风险及预后的预测价值。方法采用免疫组化MaxVision二步法观察三种蛋白在199例患者喉鳞癌组织中的表达情况，通过非条件Logistic回归及单因素、多因素生存分析，总结三者在喉鳞癌复发风险预测及预后评估中的价值。结果三种蛋白分别在低分化、颈淋巴转移及临床晚期（Ⅲ＋1V）喉鳞癌组织中表达增强趋势（P值均〈0．05），且三者表达呈正相关趋势（P值均〈0．001）。三种蛋白分别在复发组中的表达强度高于未复发组（X^2值分别为42．479，43．673和22．261，P〈0．05）。三种蛋白联合高表达患者中复发病例构成比为69．1％，高于其他联合表达模式组中的构成比（P〈0．001），且Fascin-1（比值比为7．89，95％可信区间为2．26～27．53，P=0．001）及Ezrin（比值比为2．51，95％可信区间为1．18～5．32，P〈0．001）为影响喉鳞癌复发的独立危险因素。三种蛋白分别高表达患者的5年无瘤生存率均降低（P〈0．05），而三者联合高表达患者5年无瘤生存率更低，为26．4％（P〈0．05）。三种蛋白均为影响患者预后独立危险因素（P〈0．05）。结论Fascin-1，Ezrin，Paxillin不仅与喉鳞癌的恶性进展相关，还与复发风险及预后不良相关。联合检测三者表达情况，有助于喉鳞癌复发风险预测及预后评估。
Objective To study the expressions of key assemblies of cytoskeleton, Fascin-1, Ezrin and Paxillin, in laryngeal squamous cell carcinoma （LSCC） and their correlation with clinicopathologic characteristics, cancer recurrence and survival of patients with LSCC. Methods The expressions of Fascin-1, Ezrin and Paxillin proteins were detected by immunohistochemistry in 199 cases of LSCC. Unconditional Logistic regression model or Cox proportional hazards model was used for the analyses of recurrent risks and prognostic factors. Results Significantly increased expression of Fascin-1, Ezrin or Paxillin expression was showed in the LSCC with poorly differentiated, positively cervical lymph nodal metastasis, and clinical stage HI ＋ 1V respectively （ P 〈 0. 05 ）. The expressions of three kinds of proteins in the recurrent cases were higher than those in non-recurrent cases respectively （ X2 were 42. 479, 43. 673 and 22. 261, P 〈0. 05）. The highest recurrence rate（69. 1% ） was observed in group of cases with the highly co-expresslon of the three kinds of proteins（P 〈 0. 05）. The expression of Fascin-1 （ OR --7.89, 95% CI 2. 26 - 27.53, P = O. 001 ） , or Ezrin （ OR = 2. 51, 95% CI 1. 18 - 5.32, P 〈 O. 001 ） was independent risk for recurrence. Five-year disease-free survival rates of patients with high expression of Fascin-1, Ezrin or Paxillin were lower than those of patients with negative or low expressions for the proteins （ P 〈 0. 05 ） . Patients with highly co-expression of three kinds of proteins showed the poorest survival prognosis, with a 5- year disease free survival （DFS） of only 26.4% （ P 〈 0. 05 ）, and expressions of three proteins were independent prognostic factors for 5-year DFS （P 〈 0. 05）. Conclusion Fascin-1, Ezrin, and Paxillin were correlative with LSCC progression and might be potential predictors for cancer recurrence and survival ofpatients with LSCC, as well as therapeutic targets for LSCC.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2012年第10期841-847,共7页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
国家自然科学基金（81172584）
山西省优秀研究生创新基金（20103055）
关键词
喉肿瘤
癌
鳞状细胞
载体蛋白质类
微丝蛋白质类
细胞支架蛋白质类
柱蛋白
预后
Laryngeal neoplasms
Carcinoma, squamous cell
Carrier proteins
Microfilament protein
Cytoskeletal proteins
Paxillin
Prognosis
