摘要
目的:研究FoxM1在喉鳞状细胞癌组织中的表达及其临床意义。方法:运用免疫组织化学方法检测89例喉鳞状细胞癌组织、89例癌旁组织及20例喉乳头状瘤组织中FoxM1蛋白的表达情况,分析其与喉鳞状细胞癌临床病理参数的关系。采用反转录-聚合酶链反应(RT-PCR)检测20例喉鳞状细胞癌、20例喉乳头状瘤组织以及20例癌旁组织中FoxM1mRNA表达情况。结果:FoxM1mRNA和蛋白表达的阳性率在喉鳞状细胞癌、喉乳头状瘤及癌旁组织中逐渐降低,其差异有统计学意义(P<0.05)。FoxM1蛋白的表达随组织分化程度增高而降低(P<0.05);随T分期增高而增加(P<0.05);有淋巴结转移组高于无淋巴结转移组(P<0.05),随临床分期的增加而表达增加(P<0.05)。FoxM1表达率与患者年龄、性别、癌原发部位无相关性(P>0.05)。结论:FoxM1蛋白在喉鳞状细胞癌组织中表达的上调可能与喉鳞状细胞癌的发生及浸润转移有关。
Objective：To investigate the expression of Forkhead BoxM1 transcription factor（FoxM1）mRNAand protein in laryngeal squamous cell carcinoma（LSCC） and its clinical significance. Method： Immunohistochemistry was used to examine the expression of FoxM1 in 89 LSCC tissues, 89 adjacent normal tissues and 20 laryngeal papilloma（LP） tissues. Reverse transcriptase polymerase chain reaction（RT-PCR）was used to examine the expression of FoxM1 in 20 LSCC tissues, 20 LP tissues and 20 adjacent normal tissues. The relationship between FoxM1 expression and the clinicopathological parameters of LSCC were analyzed. Result： FoxM1 mRNA and protein expression were gradually decreased from LSCC to LP and normal tissues. The difference was significant（P〈0.05）. There was a significantly correlation between FoxM1 and histologic differentiation, T stage, lymph node metastasis and clinical stage. Tumors with poorer differentiation, in higher T stage, with lymph node metastasis or in higher clinical stage presented higher FoxM1 expression than tumors with better differentiation, in lower T stage, without lymph node metastasis or in lower clinical stage（P〈0.05）. The positive expression of FoxM1 was not related with the age, gender or primary site of LSCC patients（P〉0.05）. Conelusion.. The up-regulation of FoxM1 may play an important role in the invision and metastasis of LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2012年第19期897-900,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
喉肿瘤
免疫组织化学
反转录-聚合酶链反应
FOXM1
laryngeal neoplasms
immunohistochemistry
reverse transcriptase polymerase chain reaction
FoxM1
