摘要
目的研究人非小细胞肺癌（NSCLC）组织中叉头框转录因子M1（FOXM1）蛋白的表达，探讨其与NSCLC临床病理参数及预后之间的关系。方法应用免疫组化染色法检测68例NSCLC组织中FOXM1蛋白的表达水平。选取肿瘤组织6例（FOXM1蛋白阳性和阴性表达各3例）以及正常肺组织1例，采用Western blot法检测FOXM1蛋白的表达水平，以验证免疫组化检测结果。将免疫组化检测结果与患者的临床病理特征及总生存时间（OS）进行统计学分析。结果FOXM1蛋白的阳性表达产物定位于细胞质或细胞核，其在肿瘤组织中的阳性表达率为36．8％（25／68）。Western blot法检测FOXM1蛋白的表达水平与免疫组化检测的结果一致。FOXM1蛋白在进展期NSCLC中的阳性表达率显著高于早期NSCLC（P=0．001）。FOXM1蛋白阴性和阳性表达患者的中位OS分别为23．0和13．0个月（P=0．001）。单因素分析的结果显示，NSCLC患者的预后与肿瘤的分化程度、淋巴结转移状态、分期以及FOXMI蛋白的表达情况相关（均P〈0．05）。Cox比例风险回归模型分析的结果显示，分期、淋巴结转移状态以及FOXM1蛋白的表达情况是NSCLC的独立预后因素（均P〈0．05）。结论FOXM1蛋白的表达情况是NSCLC的独立预后因素，与患者的预后呈负相关。
Objective To investigate the expression of forkhead box M1 （FOXM1） and its correlation with clinicopathological features and prognosis in patients with non-small cell lung cancer （NSCLC）. Methods The expression of FOXM1 in 68 cases of NSCLC was detected by immunohistochemistry. The FOXM1 expression in 6 tumor tissues （5 cases with negative and 3 cases with positive expression of FOXM1 ） was analyzed by Western blotting to confirm the immunohistochemical results. The correlation of the expression of FOXM1 with clinicopathalogical features and overall survival of the NSCLC patients was analyzed. Results The expression of FOXM1 protein was detected in the nuclei or cytoplasms of the tumor cells. The positive expression rate of FOXM1 was 36.8% （25/68）. Western blotting confirmed the immunohistochemical results. The expression level of FOXM1 in advanced stage cancer was significantly higher than that in early stage NSCLC （P =0.001）. The median OS was 23.0 months in patients with negative expression of FOXM1 and 13.0 months in those with positive expression （P =0. 001 ）. Univariate analysis revealed that histological grade, lymph nodes status, TNM stage and FOXMI expression were significantly associated with prognosis in the NSCLC patients （P 〈 0. 05 ）. The Cox multivariate analysis demonstrated that lymph nodes status, TNM stage and FOXM1 expression were independent poor prognostic factors （P 〈 0.05 ）. Conclusion The expression status of FOXM1 in NSCLC is an independent prognostic factor and negatively correlated with prognosis.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2011年第6期426-430,共5页
Chinese Journal of Oncology
基金
国家自然科学基金（30772549）
江苏省卫生厅医学科技发展基金（P200965）
