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趋化因子受体CXCR4在骨髓间充质干细胞中的表达 被引量：2

Expression of CXC chemokine receptor-4 in bone mesenchymal stem cells
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摘要 目的体外研究趋化因子受体CXCR4在骨髓间充质干细胞中的表达情况。方法体外培养大鼠骨髓基质细胞,构建CXCR4腺病毒表达载体,采用Western blotting对骨髓基质细胞的CXCR4受体表达水平进行检测。结果骨髓间充质干细胞能高表达CXCR4蛋白。结论 CXCR4蛋白在骨髓间充质干细胞高表达,为进一步研究SDF-1/CXCR4轴介导BMSCs在肝脏选择性归巢打下基础。 Objective To study the expression of CXC chemokine receptor-4 （CXCR4） in bone mesenchymal stem cells. Methods Bone mesenchymal stromal stem cells （BMSCs） of rats were cultured in vitro, adenovirus vecto of CXCR4 was constructed and the expression level of CXCR4 receptor of BMSCs was tested by western blot. Results BMSCs can express CXCR4 and the positive ratio was high. Conclusion The high ratio of expression laid foundations for the further study in the effect of stromal cell-derived factor-1/CXCR4 （SDF- 1/CXCR4） axis on bone mesenchymal stem cell homing to liver.
作者 朱志立 米娜 李靖 郑璐 黄小兵
机构地区 第三军医大学基础医学部外科应用解剖与手术学教研室 第三军医大学新桥医院肝胆外科
出处 《局解手术学杂志》 2010年第6期462-464,共3页 Journal of Regional Anatomy and Operative Surgery
基金 重庆市自然科学基金资助项目(2008C165)
关键词 骨髓间充质干细胞 趋化因子受体CXCR4 归巢 bone mesenchymal stem cells （BMSCs） CXC chemokine receptor-4 （CXCR4） homing
分类号 R657.31 [医药卫生—外科学] Q254 [生物学—细胞生物学]
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参考文献8

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共引文献11

同被引文献13

  • 1杜少辉,陈东风,李伊为,黎晖,周健洪,邓汝东,张赛霞.龟板对脑缺血大鼠骨髓间充质干细胞移植后转分化为神经元的影响[J].中华医学杂志,2005,85(3):205-207. 被引量：44
  • 2张刚庆,方驰华,池达智.共同培养诱导骨髓基质干细胞向肝细胞分化的研究[J].中华肝脏病杂志,2005,13(9):648-651. 被引量：13
  • 3李熙灿,谢学明,黄春花,钟远声,李伊为,周健洪,陈东风.龟板醇提物对大鼠骨髓间充质干细胞氧化损伤的修复及其抗脂质过氧化作用[J].中草药,2007,38(7):1043-1046. 被引量：18
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引证文献2

局解手术学杂志
2010年 第6期

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