摘要
目的评价大豆异黄酮(SI)对动物生殖发育的影响。方法 4周龄SD大鼠雌雄各20只。SI组喂饲100mg/kg BW的添加SI的饲料,对照组喂饲普通饲料。检测亲代体重、动情周期、血清激素、精子质量;F1代出生体重及身长、性别比、肛门生殖孔间距、阴道开口和包皮分离时间等。两独立样本t检验比较均数,子代数据以每窝为单位。结果与对照组相比,SI组亲代雌鼠交配前体重和雌雄鼠的食物利用率较低;亲代雄鼠精子总数较高,血清促黄体生成素较低;亲代雌鼠促黄体生成素、催乳素和孕酮的水平较低(以上P<0.05);其他指标在两组中无显著差异。结论 SI对亲代雌鼠交配前的体重、雌雄鼠的食物利用率、精子总数、血清部分激素水平等有影响。
Abstract：Objective To assess the effects of soybean isoflavone on reproductive development in SD rats. Methods Forty 4 week-old SD rats （20 males and 20 females） were randomly divided into a control group and a soy isoflavone （SI） group according to their body mass, and were fed with normal diet or normal diet added with soy isoflavone （100mg/kg BW）, respectively. The body weight, daily food intake, estrous cycle, fertility index, pregnant index, gestation length, number of pups, blood hormone level, the weight of reproduction organ and the quality of sperm of the parental generation and the sex ratio, anogenital distance, vagina opening time, prepuce separation time of F1 generation were recorded. The data were analyzed by two independent t test, and the data of pups in each litter were analyzed as a unit. Results Compared with the control group, the body weight and food utilization of male and female rats in SI group were lower before copulation （P 〈 0.05）. The total number of sperm of parental males was higher （P 〈 0.05）. Serum luteinizing hormone （LH） level of parental male was lower （ P 〈 0.05 ） , serum luteinizing hormone （LH）, prolactin （PRL） and progesterone （P） of parental female were lower in SI group （P 〈 0.05）. There were no significant differences of other indices such as estrous cycle, mating index, fertility index between two groups.Conclusion SI has certain effect on the bodyweight of parental males, food utilization of females, and the total number of sperm and the level of some hormones.
出处
《卫生研究》
CAS
CSCD
北大核心
2012年第5期730-734,共5页
Journal of Hygiene Research
基金
国家自然科学基金项目(No.81172653
30872115)
关键词
大豆异黄酮
繁殖实验
生殖
生长发育
soy isoflavones, reproduction study, growth and development
