摘要
用荞麦无菌苗的幼茎和幼叶作外植体分别接种在MS补加不同激素组合的培养基上培养获得再生植株 实验结果表明 ,适宜诱导愈伤组织的激素组合为 2 4-D 4 0mg/L +KT0 2mg/L ,适宜愈伤组织生长的激素组合为 2 4-D 1 0mg/L +KT 1 0mg/L 诱导芽的较适宜的组合为 6 -BA 3 0mg/L +IAA 0 1mg/L 不定芽接种到含MS+IBA 2 0mg/L+KT 0
Stem segments and leaf segments of Buckwheat were cultured on MS medium supplemented with different phytohormone compositions and the plant regrneration was obtained The experiment's results showed that 2 4-D was necessary for inducting callus of buckwheat The suitable phytohormone composition to induct callus was 2 4-D 4 0mg/L + KT 0 2mg/L The suitable phytohormone compsition for callus growing was 2 4-D 1 0mg/L + KT 1 0mg/L; It was very difficult to induce the adventitious buds from the callus kept on 2 4-D + KT medium The suitable medium to induce adventitious buds and roots were 6-BA 3 0mg/L + IAA 0 1mg/L MS and IBA 2 0mg/L + KT 0 1 mg/L MS respectively
出处
《湖北三峡学院学报》
2000年第2期64-66,69,共4页
Journal of China Three Gorges University(Humanities & Social Sciences)
