摘要
采用超临界CO2流体萃取灵芝孢子油,在压力30 MPa和温度40℃条件下进行萃取(CO2流量25 L/h,时间2.0 h),在8 MPa和45℃条件下进行分离,灵芝孢子油的平均得率为24.35%,提取率达95.08%。高效液相色谱分析结果表明:灵芝孢子油中的脂溶性维生素D2、维生素D3、维生素E的含量分别为15.13、14.68、7.15μg/g。体外抗氧化活性检测结果表明:灵芝孢子油具有较强的DPPH自由基和超氧阴离子自由基清除能力,并且呈明显的剂量效应关系;当样品质量浓度达800μg/mL时,对DPPH自由基和超氧阴离子自由基的清除率可达89.1%和41.3%,较接近于抗氧化剂BHT和芦丁;但对ABTS自由基的清除作用较弱。
Ganoderma lucidum spores oil was collected from the separator（ at 8 MPa, 45 ℃ ） by supercrit- ical CO2 fluid extraction at 30 MPa and 40 ℃, with 25 L/h of CO2 flux for 2.0 h, and the averaged yield was 24.35% while the extract rate reached to 95.08%. High performance liquid chromatography analy- sis showed that the content of vitamin DE, D3and E in the spores oil were 15.13, 14.68 μg/g and 7.15 μg/g. The spores oil had positive antioxidant activity in vitro, which were mostly related to the tested samples concentrations. When the concentration of spores oil was 800 μg/mL, its scavenging ability on DPPH radical and superoxide radical were 89.1% and 41.3% , which were near to the degree of BHT and rutin, respectively; its scavenging effect on ABTS free radical was not obvious.
出处
《中国油脂》
CAS
CSCD
北大核心
2012年第9期48-50,共3页
China Oils and Fats
基金
福建省农业科学院创新团队重点项目(CXTD-2011-24)
关键词
灵芝孢子油
脂溶性维生素
抗氧化活性
Ganoderma lucidum spores oil
lipid- soluble vitamins
antioxidant activity
