摘要
目的观察葡萄籽原花青素(GSP)对肾血管性高血压(RH)大鼠血管内皮细胞(VEC)功能的影响。方法采用两肾一夹(2K1C)法复制RH大鼠模型,并设假手术组(control,n=8)。术后2周,选取鼠尾动脉收缩压升至130 mmHg以上的大鼠32只为RH大鼠,随机分为4组(n=8):高血压模型组(RH model)、GSP低剂量治疗组[low GSP,50 mg/(kg·d)]、GSP高剂量治疗组[high GSP,200 mg/(kg·d)]和卡托普利阳性对照治疗组[captopril,30 mg/(kg·d)]。治疗6周后,分别测定各组大鼠尾动脉收缩压、血清中内皮素(ET)、前列环素(PGI2)和血管紧张素Ⅱ(AngⅡ)含量,Western blotting法检测腹主动脉中内皮型一氧化氮合酶(eNOS)的蛋白表达。结果治疗6周后,与control组相比,RH model组大鼠的尾动脉收缩压、血清中ET和AngⅡ含量明显升高,而血清中PGI2含量和腹主动脉中eNOS的蛋白表达明显降低;与RH model组相比,GSP各剂量治疗组和captopril治疗组大鼠的尾动脉收缩压、血清中ET和AngⅡ含量显著降低,血清中PGI2含量和腹主动脉中eNOS的蛋白表达显著升高。结论 GSP能降低RH大鼠尾动脉收缩压、减少RH大鼠血清中缩血管物质ET和AngⅡ含量、增加舒血管物质PGI2含量及增强腹主动脉中eNOS的蛋白表达进而发挥保护VEC功能的作用。
【Objective】 To observe the effects of grape seed procyanidin（GSP） on the vascular endothelial cells function in renovascular hypertensive（RH） rats.【Methods】 The RH rat model was established by 2K1C method,8 rats with sham operation were served as controls.Two weeks after operation,32 rats were selected according to the increased tail systolic pressure above 130 mmHg and randomly divided into 4 groups（n =8）： renovascular hypertensive model group（RH model）;low GSP-treated group [low GSP,50 mg/（kg·d）];high GSP-treated group [high GSP,200 mg/（kg·d）] and captopril-treated group [captopril,30 mg/（kg·d）].The tail systolic pressure,endothelin（ET）,prostaglandin I2（PGI2） and angiotensin Ⅱ（Ang Ⅱ） levels in serum were determined 6 weeks after treatment.Western blotting was performed to detect the expression of endothelial nitric oxide synthase（eNOS） in abdominal aortic tissues.【Results】 6 weeks after treatment,compared with control group,the tail systolic pressure,ET and Ang II levels in serum in RH model group were increased significantly,but PGI2 level in serum and the expressions of eNOS in abdominal aortic tissues were decreased.Compared with RH model group,the tail systolic pressure,ET and Ang Ⅱ levels in serum in low and high GSP-treated group and captopril-treated group were decreased markedly,but PGI2 level in serum and the expressions of eNOS in abdominal aortic tissues were increased significantly.【Conclusion】 GSP possesses a protecting role of vascular endothelial cells function in RH rats by reducing tail systolic blood pressure,decreasing the vasoconstrictor substances ET and Ang Ⅱ levels in serum,increasing vasodilator substances PGI2 content and eNOS expression in the abdominal aorta.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第23期23-26,共4页
China Journal of Modern Medicine
基金
广东省珠海市科技局资助项目(No:PC20071045)
贵州省科技厅资助项目(No:黔科合J字[2008]2317)
贵州省高层次人才科研条件特助经费资助项目(No:TZJF-2008年58号)
