摘要
目的:观察丹酚酸B(salvianolic acid B,Sal B)对心肌缺血大鼠缺血心肌血管新生的作用。方法:选取SD大鼠60只,结扎大鼠结扎大鼠冠状动脉左降支,建立大鼠心肌缺血模型,随机分为模型组,Sal B 1.6,3.2,6.4 mg.kg-1组,和血管内皮生长因子(VEGF)组(10μg/只),并设立假手术组,每组10只,给药2周后,HE染色法检测心肌梗死面积;试剂盒检测心肌组织中一氧化氮(NO)、一氧化氮合酶(NOS)和VEGF含量;免疫组化法检测大鼠缺血心肌中微血管数(MVC)。结果:Sal B3.2,6.4 mg.kg-1组梗死面积(7.18±3.15)%,(5.74±2.02)%较模型组(14.84±5.40)%明显减小;Sal B能增加心肌组织中NO,NOS,VEGF含量;Sal B 3.2,6.4 mg.kg-1组心肌梗死边缘区MVC(28.6±5.86),(30.20±5.07)条,较模型组(15.60±4.62)条,明显增多。结论:Sal B可促进心肌缺血后大鼠缺血心肌血管新生,其作用机制与VEGF、NO释放增加有关。
Objective： To observe the effect of salvianolic acid B （Sal B） on the angiogenesis in ischemic myocardium of the rats with myocardial ischemia. Method： The left coronary of SD rats were blocked to make the models of acute myocardial ischemia. Sixty rats were randomized into model group, Sal B groups （ 1.6, 3.2, 6.4 mg kg-1） and vascular endothelial cell growth factor （VEGF） group. A sham operation group was set up. Ten rats were included in each group. Two weeks after administration, HE staining was used to detect the myocardial infarct size; using kits to detect the contents of nitrogen oxide （NO）, NO synthase （NOS） and VEGF in myocardial tissue; immunohistochemical method was employed to detect microvessel counts （MVC） in ischemic myocardium. Result： The infarct size of myocardium in Sal B 3.2, 6.4 mg .kg-1 groups （7.18 _＋ 3.15）%, （5.74 ＋ 2.02） % was lower than that in the model group （ 14.84 ＋ 5.40） ; Sal B could increase the contents of NO, NOS and VEGF in myocardial tissue. The number of MVC in the marginal zone of myocardial infarct in Sal B 3.2, 6.4 mg ·kg-1 groups （28.6 ± 5.86）, （30.20± 5.07） was higher than that in the model group （ 15.60 ± 4.62）. Conclusion： Sal B may promote angiogenesis of ischemic myocardium in the rats with myocardial ischemia. Its mechanism might be related to promoting the contents of VEGF and NO.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期180-183,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部"十二五"重大新药创制科技重大专项(2011ZX09102-002-07)
