摘要
目的:优选蒙古黄芪提取物中总皂苷的精制工艺。方法:以蒙古黄芪总皂苷为指标,紫外分光光度法测定其含量,单因素试验考察吸附容量、洗脱溶剂及其用量;选取上样量、吸附时间和洗脱流速为考察因素,采用正交试验法优选其洗脱条件。结果:优选的精制工艺为上柱溶液5 mL加10 mL水混匀,上柱,吸附时间1.0 h,加10倍量水洗脱,5倍量70%乙醇洗脱,收集洗脱液,洗脱流速2 mL.min-1。洗脱液减压回收至干,于105℃干燥后总固体物中总皂苷纯度达到87.63%。结论:该优选工艺简单可行,能较好地纯化蒙古黄芪总皂苷。
Objective： To optimize refining process of total saponins from extracts of Astragalus membranaceus. Method： With total saponins from A. membranaceus as index, the content of it was determined by UV spectrophotometry, adsorption capacity, kind and its amount of elution solvent were investigated by single factor test; Sample quantity, adsorption time and elution flow velocity were chosen as factors, elution conditions were optimized by orthogonal test. Result： optimized refining process was： mixed with 5 mL sample solution and 10 mL water, on column, adsorption time 1.0 h, eluted with 10 times the amount of water and 5 times the amount of 70% ethanol, collected eluent, elution flow velocity 2 mL.min-1 Eluent was decompressed recovery to dried, purity of total saponins was up to 87.63% after drying at 105 ~C. Conclusion： This optimized technology was simple and feasible, it could be better to purify total saponins from A. membranaceus.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期55-57,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
大孔树脂
蒙古黄芪
总皂苷
精致工艺
macroporous resin
Astragalus membranaceus
total saponins
refining process
