摘要
目的:比较不同型号大孔吸附树脂对酸枣仁皂苷的纯化效果,并优选其纯化工艺。方法:以酸枣仁皂苷A为指标成分,HPLC-ELSD测定指标成分含量。通过静态吸附法和动态吸附法优选大孔树脂型号,采用正交试验和单因素试验考察其纯化工艺。结果:酸枣仁皂苷A在0.216～2.16μg与峰面积成良好线性关系,平均加样回收率为95.77%,RSD 0.71%。ADS-7型大孔吸附树脂能有效除去色素,纯化效果最好,其优化纯化工艺为柱高径比3:1,药液质量浓度1.0 g.mL-1,吸附速度1.0 BV.h-1,树脂生药吸附量0.4 g.g-1,洗脱溶媒70%乙醇,洗脱速度2 BV.h-1,洗脱溶媒用量4 BV。结论:ADS-7型大孔吸附树脂对酸枣仁皂苷A纯化效果较好,工艺稳定可行,可用于工业化生产。
Objective： To compare purification effect of saponins from Ziziphus jujube with different types of macroporous adsorption resin; and to optimize its purification technology. Method： With jujuboside A as index, the content of it was determined by HPLC-ELSD. Types of macroporous resin were optimized by static adsorption and dynamic adsorption method, purification technology of it was investigated by orthogonal test and single factors test. Result： For jujuboside A, there was a good linear relationship in the range of 0. 216-2.16 μg, average recovery was 95.77% , RSD was 0.71%. ADS-7 macroporous resin could effectively remove pigment with better purification effect of total saponins than other resins, optimum technological parameters were： column height-diameter ratio of 3 ： 1, the concentration of liquid 1.0 g ·mL-1, absorption velocity 1.0 BV ·h^-1, resin adsorption quantity 0.4 g· g-1, elution solvent 70% ethanol, elution speed 2 BV·h^-1 , elution solvent volume 4 BV. Conclusion： ADS-7 macroporous resin had a good purification effect on jujuboside A, optimized technology was stable, feasible, it could be used for industrial production.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期63-66,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
正交设计
酸枣仁
大孔吸附树脂
纯化工艺
orthogonal design
Ziziphus jujube
macroporous adsorption resin
purification technology
