摘要
绿色荧光蛋白（ＧＦＰ）是海洋生物水母（Ａｅｑｕｏｒｅａｖｉｃｔｏｒｉａ）体内的一种发光蛋白，分子量２７ｋＤ，由２３８个氨基酸组成。该蛋白６５～６７位ＳｅｒＴｙｒＧｌｙ三种氨基酸环化加氧形成特殊的生色团结构。野生型ＧＦＰ发光较弱，而且ｇｆｐｃＤＮＡ含有隐蔽型剪切位点，而加工改造的ＧＦＰ在植物中能够正常表达并且加强了荧光信号。ＧＦＰ作为新的报告基因和遗传标记被广泛应用于植物研究之中。
Green fluorescent protein (GFP) from the jelleyfish Aequorea victoria is responsible for the bioluminescence. The Aequrea GFP is composed of 238 aa residue with a calculated M r of 26888D. The highly fluorescent chromophore is composed of a modification of the Ser Tyr Gly at positions 65～67 within the polypeptide. These three residues are cyclized and oxidized to form the phydroxybenzylidene imidazo lidinone chromophore. The expression of wild type gfp in stably transformed plants has typically yielded very faint or no green fluorescence. The engineered gfp gene was resynthesized to adapt its codon usage for expression in plant by increasing G or C content to mutate the cryptic intron for escaping the aberrant mRNA processing. The modified gfp versions were used as a reporter gene in place of GUS gene, as a marker gene and as a subcellular targeting tool in plant. In addition, GFP was effective in the research of ecological monitoring and interactions between microbes and plants.
出处
《植物学通报》
CSCD
1998年第5期24-30,共7页
Chinese Bulletin of Botany
关键词
绿色荧光蛋白
GFP基因
植物
Green fluorescent protein (GFP), GFP gene, Plant
