摘要
目的:采用毛细管气相色谱建立八角茴香油中草蒿脑的含量测定方法。方法:通过水蒸气蒸馏从八角茴香中提取得挥发油,采用毛细管气相色谱进行分析测定。HP-INNOWax毛细管柱(0.32 mm×30 m,0.50μm),分流比100∶1程序升温,FID检测器。结果:草蒿脑在0.08～0.32μg峰面积与进样量呈良好线性关系(r=0.999 8),草蒿脑定量限为2.0 ng,平均回收率为101.0%,RSD 1.27%(n=9)。结论:该方法简便、准确、重复性好,可作为八角茴香油中草蒿脑的质量控制方法。
Objective：To establish a method for the determination of estragole in anise oil.Method：The essential oil was extracted from Illicium verum and then analyzed by GC.the GC was fitted with HP-INNOWax capillary column（0.32 mm×30 m×0.50 μm） film thickness;flame ionization detector and operated under the following conditions： Nitrogen carrier gas;split injection 100∶1;temperature programming.Result： The calibration curves were linear in the range of 0.08-0.32 μg for estragole,r=0.999 8,average recovery of estragole was 101.0%,RSD was 1.27%（n=9）.Conclusion： The method is simple,accurate,reproducible and can be used as the quality control for Illicium verum.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第19期89-91,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技成果推广与产业化示范项目(桂科产0915010-7E)
