摘要
目的:研究因宁片对甲亢性肝损害模型大鼠肝脏细胞色素P450(CYP450)基因表达的影响。方法:采用L-甲状腺激素钠灌胃以复制甲亢性肝损害大鼠模型。实验分为6组,即空白对照、模型、甲亢灵和因宁片高、中、低剂量(2.4、1.2、0.6g.kg-1)组。通过实时荧光定量聚合酶联反应(PCR)方法检测因宁片对肝组织内CYP1A2、CYP2E1和CYP3A2基因表达的影响。结果:与模型组比较,因宁片高、中剂量组大鼠肝脏CYP2E1的表达显著减弱(P<0.05),CYP1A2和CYP3A2的表达无明显改变(P>0.05)。结论:因宁片能下调甲亢大鼠肝脏CYP2E1基因的表达,这可能是因宁片治疗甲亢性肝损害的分子作用机制之一。
OBJECTIVE：To study the effects of Yinning tablet on gene expression of cytochrome P 450（CYP 450）in liver tissue of rats with hyperthyroidism liver damage.METHODS：Rats were perfused with levothyroxine sodium intragastrically to establish the rat model of hyperthyroidism liver damage.The model rats were divided into 6 groups,i.e.blank control group,model group,Jiakangning group and Yinning tablet high-dose,medium-dose and low-dose groups（2.4,1.2,0.6 g.kg-1）.The expressions of CYP1A2,CYP2E1 and CYP3A2 in liver tissue were detected by real-time quantitative PCR.RESULTS：Compared with model group,the expressions of CYP2E1 was decreased significantly（P〈0.05）,and the expressions of CYP1A2 and CYP3A2 had no significant change（P〉0.05）.CONCLUSION：Yinning tablet may down-regulate the expression of CYP2E1 in liver tissue of rats with hyperthyroidism liver damage,which may be one of the molecular action mechanisms that Yinning tablet prevent hyperthyroidism liver damage.
出处
《中国药房》
CAS
CSCD
2012年第39期3667-3669,共3页
China Pharmacy
