摘要
以苹果轮纹病菌、苹果腐烂病菌、苹果黑斑病菌、苹果斑点落叶病菌和苹果灰霉病菌为供试菌种,采用生长速率法和孢子萌发法测定嗜线虫致病杆菌HB310的发酵液和上清液对病原菌菌丝生长发育的抑制作用及对孢子萌发的影响。结果表明:嗜线虫致病杆菌HB310发酵液和上清液对苹果轮纹菌菌丝生长的抑制效果最好,抑制率分别为76.90%和80.79%。嗜线虫致病杆菌HB310发酵液致使苹果腐烂病原菌菌丝形态异常;浓度为50和25mL/L的嗜线虫致病杆菌HB310对供试苹果病原真菌的孢子萌发均有很好的抑制作用,抑制率均在98%以上。嗜线虫致病杆菌HB310对苹果病原真菌的菌丝和孢子均具有较好的抑制作用。
To explore the antimicrobial activity of the culture broth and supernatant of Xenorhabdus nematophila HB310 against five apple pathogens.The antimicrobial activity of the culture broth and supernatant of Xenorhabdus nematophila HB310 against Botryosphaeria berengeriana,Valsa mali,Alternaria mali,Botrytis cinerea and Alternaria mali by using two methods-growth rate method and spore germination method were investigated.The results showed that the inhibition of culture broth and supernatant of Xenorhabdus nematophila HB310 to mycelium growth of Botryosphaeria berengeriana were 76.90% and 80.79%.Overstriking mycelia and malformed cell membrane were were observed in V.mali after treatmented by the X.nematophila HB310.In addition,the X.nematophila HB310 also inhibited the spore germination.When the X.nematophila HB310 were on 50 mL/L and 25 mL/L,the spore germinations of three apples fungi were above 98%.The antimicrobial activity of the culture broth and supernatant of Xenorhabdus nematophila HB310 against five apple pathogens both had better effect.
出处
《北方园艺》
CAS
北大核心
2012年第20期123-126,共4页
Northern Horticulture
基金
国家苹果产业技术体系资助项目(nycytx-08-04-01)
国家公益性农业行业资助项目(200903004-42)
关键词
嗜线虫致病杆菌
苹果病原真菌
抑菌活性
Xenorhabdus nematophila HB310
apples fungi
antimicrobial activity
