摘要
用本实验室已建立的贝类组织培养技术对马氏珠母贝外套膜组织成功地进行了体外培养。在外套膜组织中最先迁出的是颗粒细胞 ,紧随其后为透明细胞 ,在培养到 2 0 h时 ,圆形的上皮细胞开始迁出 ,上皮细胞很快在组织块周围形成生长晕 ,继而铺满整个培养瓶底面 ;培养 4d以后 ,上皮细胞开始分泌颗粒状的物质 ,这时的上皮细胞从形态上可分为 A型和 B型两类 ,B型上皮细胞含有许多颗粒物质 ,而 A型上皮细胞不含或含少量颗粒物质 ,反映了其合成分泌物的状态。上皮细胞活跃的活动能持续很长时间 ,分泌物可在培养瓶内结块。上皮细胞在体外培养存活达 6 5 d左右。在外套膜组织的培养中 ,还出现了大量的棱形肌肉细胞。本研究同时对外套膜愈伤组织进行培养 。
Mantle tissue of the pearl oyster,Pinctada martensii was cultured in vitro for periods of up to 65 days. After 2.5 hours of culture, roundish granulocytes began to migrate out from the mantle tissue explant. This was followed by the hyalinocytes or agranulocytes with pseudopodia. After 20 hour culture, the epithelial cells migrated and within a short time had formed growth sheets in the periphery of the explant and on the surface of the flask. Four days after initiation of the culture, the entire surface of the flask was covered by a cell sheet mostly consisting of the epithelial cells. At this time, epithelial cells were of two distinct morphologically types, epithelial cell A (ECA) and epithelial cell B (ECB). Both types of epithelial cells appeared to secret fine nacreous matter, although ECA secreted considerably more nacre than ECB. The secretive activity of the epithelial cells continued until pieces of nacreous matter eventually formed on the flask. During culture, elongate, spindle like muscle cells also appeared. In this study, lacerated mantles were also cultured which was found to reduce the time before epithelial cells emigrated into the culture.
出处
《广西科学》
CAS
2000年第2期135-139,共5页
Guangxi Sciences
基金
国家自然科学基金!( 3 95 60 0 66)
广西自然科学基金匹配资助项目!( 962 40 10 )
