摘要
通过对三角帆蚌外套膜上皮组织块培养和上皮细胞培养在包硬核、无包硬核的情况下的钙代谢进行比较分析,用偏光显微镜观察。结果发现,外套膜上皮组织块、上皮细胞在有硬核时与对照的无硬核培养钙含量无明显差别,外套膜组织块经培养迁移和增值,能形成珍珠囊的上皮细胞,其结缔组织细胞共同围绕珍珠囊上皮细胞组成完整的珍珠囊。偏光显微镜下能观察到双折射现象,表明产生由碳酸钙结晶形成的片层叠加构成珍珠质。该研究进一步探讨了体外培育珍珠(试管珍珠)的技术,为试管珍珠的研究提供了实验依据。
By using polarizing microscope, the calcium metabolism of the cultured epithelial tissue and cells with hard nucleus-insertion or not of the mantle from Hyriopsis cumingii had been analyzed. The results showed that no differences in the calcium content were found between two different conditions. Whereas, the cultured mantle tissue proliferated and evolved to the epithelial cells of pearl-sac, the integrated pearl-sac was formed by the approach of the connective tissue encircling the epithelial cells of pearl-sac. Otherwise, birefringence phenomena was observed, which indicated that the calcium carbonate crystal made up of the nacre through lamellar accumulation. The study also discussed the technique of pearl culture in vitro （Tube pearl）, which provided the data for the further experiments.
出处
《水生态学杂志》
北大核心
2009年第1期74-77,共4页
Journal of Hydroecology
基金
浙江省农业科技重大攻关项目(2006C22030)
关键词
外套膜
硬核
钙含量
珍珠囊
mantle
hard nucleus
calcium content
pearl-sac
