摘要
分别测定了打瓜外皮、内皮、瓤和籽中的蛋白质和可溶性总糖,再提取各部位多糖并测定其自由基清除活性。实验结果表明,在干打瓜外皮、内皮、瓤和籽中蛋白质含量分别为6.65%、1.22%、5.60%和30.04%。在鲜瓜外皮中可溶性糖含量为33.94mg/g,鲜内皮中为29.77mg/g,鲜瓤中为58.94mg/g,干籽中为32.95mg/g。用醇沉法由鲜外皮、内皮、瓤和干籽中所得的提取物中多糖含量分别为33.29%、28.05%、93.39%和15.77%,鲜外皮、内皮、瓤和干籽中多糖含量分别为0.17%、0.016%、0.19%和1.59%。外皮、内皮、瓤和籽的水溶醇沉提取物对羟基自由基、超氧阴离子和DPPH自由基具有一定的清除能力。其中打瓜外皮、内皮、瓤和籽提取物对羟基自由基清除,以及打瓜内皮提取物对DPPH的清除能力好于同浓度维生素C。
The contents of protein and total soluble carbohydrate were determined in rind,endodermis,pulp and seeds of seed melon. PolysaccharJde was isolated from the sections and the radicals scavenging activities were evaluated. The experimental results showed that the contents of protein were 6.65% in dried rind, 1.22% in dried endodermis,5.60% in dried pulp and 30.04% Jn dried seeds,the contents of soluble carbohydrate were 33.94mg/g in fresh rind,29.77mg/g in fresh endodermis,58.94mg/g in fresh pulp and 32.95mg/g in dried seeds,respectively. The extracts were isolated from fresh rind,endodermis and pulp by ethanol-precipitating, from dried seed by water-dissolving and ethanol-precipitating,the polysaccharide contents in the extracts were 33.29% from fresh rind,28.05% from fresh endodermis,93.39% from fresh pulp and 15.77% from dried seed. The polysaccharide contents in the different sections of the melon were 0.17% in fresh rind,0.016% in fresh endodermis,0.19% in fresh pulp and 1.59% in dried seed. The extracts showed certain antioxidant activities on the radicals of hydroxyl,superoxide and DPPH. The antioxidant activities of the extracts from rind, endodermis,pulp and seed on hydroxyl radical and the extract from endodermis on DPPH were better than vitamin C.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第20期105-108,共4页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31172009
30972038)
关键词
打瓜
蛋白质
可溶性总糖
水溶性多糖
自由基清除活性
seed melon
protein
total soluble carbohydrate
water-soluble polysaccharide
radicals scavenging activities
