摘要
目的研究医院梅毒酶联免疫吸附试验(ELISA)筛查阳性标本患者的年龄分布,分析ELISA与苍白密螺旋体明胶颗粒凝集试验(TPPA)在梅毒检测中的价值。方法 ELISA法对住院患者进行梅毒抗体初筛,双孔复查仍为阳性者,进一步进行TPPA确证;对ELISA阳性的病例按S/CO值分为灰区组和阳性组,分别与TPPA方法进行比较,结果进行统计处理。结果共检出ELISA阳性113例,与TPPA方法的阳性符合率为93.81%,>60岁患者阳性率为5.00%,占总阳性患者的46.90%;灰区组、阳性组运用ELISA及TPPA比较,检测结果差异无统计学意义。结论梅毒血清学试验有一定的假阳性率,高龄患者中尤为突出,当ELISA检测结果与患者病史及临床表现不一致时,应尽早复查和TPPA确证,同时定期随访。
OBJECTIVE To investigate the age distribution of the positive cases screened by syphilis enzyme-linked immunosorbent assay（TP-ELISA）,and evaluate Treponema pallidum ELISA and gelatin particle agglutination test（TPPA） in the detection of syphilis.METHODS The preliminary screening of positive cases was conducted with syphilis antibody ELISA for the inpatients.The further confirmation of TPPA method was conducted for the cases whose double-check results remained positive.The ELISA-positive cases were divided into the gray-area group and positive group according to S/CO values,and the two groups were statistically compared with those of TPPA analysis respectively.RESULTS Totally 113 positive cases were found using ELISA method.Among them,93.81% of cases could also be screened with TPPA analysis;the positive rate was 5.00% for the case group of over sixty-year old inpatients which accounted for 46.90% of total positive cases.No statistical difference could be found between ELISA-positive cases and TPPA-positive cases in both gray-area group and positive group according to S/CO values.CONCLUSION False-positive cases exist in the syphilis serology tests,especially among the very old people.When the result of ELISA test is inconsistent with the medical history and clinical manifestation of the patients,re-examination and confirmation by TPPA test should be carried out as soon as possible,meanwhile,regular follow-up is needed.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第20期4664-4665,共2页
Chinese Journal of Nosocomiology
关键词
梅毒
梅毒抗体
梅毒酶联免疫吸附试验
苍白密螺旋体明胶颗粒凝集试验
Syphilis
Syphilis antibodies
Enzyme-linked immunosorbent assay
Gelatin Treponema pallidum particle agglutination test
