摘要
目的 :为了探讨GST -π在食管癌发生、发展中的作用及其对食管癌早期诊断的意义。方法 :应用抗GST -π多克隆抗体 ,以石蜡切片LSAB免疫组化方法对 42例食管鳞状细胞癌及相应的癌旁组织进行检测。结果 :①GST -π在不典型增生、原位癌 ,Ⅰ级鳞癌的细胞核内高表达 ,明显高于正常粘膜。②在癌旁组织的底层中 ,仅原位癌的核表达阳性率与正常粘膜相比具有显著差异 (P <0 .0 1 ) ;在中层和表层 ,不典型增生和原位癌的GST -π核表达与正常和单纯性增生相比均有显著差异 (P <0 .0 2 5)。结论 :GST -π可能是食管癌发生的一个早期酶学标志物 。
Objective:To explore the relationship between GST-π and the genesis and progression of esophageal carcinoma(EC),and the significance of GST-π in the early diagnosis of EC.Methods:Esophageal squamous cell carcinoma and paracancerous tissues were investigated immunohistochemically with labelled streptavidin biotin method(LSAB) using polyclonal anti-GST-π antibody,Paraffin sections of esophagectomy specimens fixed in formalin .Results:The positive rates of nuclear expression of GST-π in normal esophageal epithelia,simple hyperplasia,mild dysplasia,severe dysplasia,carcinoma in situ and in Grade I,Grade Ⅱ,Grade Ⅲ of esophageal squmaous cell carcinoma were 13.5%、20.5%、44.8%、39.1%、60%、58.3%、32% and 0% respectively.The others were significantly higher than that in normal group,except those in simple hyperplasia and Graed Ⅱ or Grade Ⅲ(P<0.025).Campared with that in the normal epithelia,the positive rates of nuclear staining of GST-π were not statistically signficant in the basal layer of paracancerous tissue except that in carcinoma in situ(P<0.01).In the intermediate or the superficial layers,the positive rates of nuclear expression of GST-π in dysplasia and carcinoma in situ were signficntly higer than those in normal and simple hyperplasia epithelia (P<0.025).Conclusion The overexpression of GST-π has already appeared in simple hyperplasia,which suggested that the GST-π is an early event and perhaps is an early marker in carcinogenesis of esophagus.GST-π might be a useful marker of esophageal carcinoma and might be valuable in the early diagnosis of esophageal carcinoma and in the finding of high-risk masses of people.
出处
《长治医学院学报》
2000年第2期81-83,共3页
Journal of Changzhi Medical College
